| Transactions |
| Date | Team | Player | Type | OthTm | EffDate | When |
| 4/2/2016 | Pit | Cabrera,Mi | Acquired via trade | Tex | | After |
| 4/2/2016 | Pit | Caminero | Acquired via trade | Tex | | After |
| 4/2/2016 | Pit | Carter | Traded away | Tex | | After |
| 4/2/2016 | Pit | Cingrani | Acquired via trade | Tex | | After |
| 4/2/2016 | Pit | Frazier,C | Traded away | Tex | | After |
| 4/2/2016 | Pit | Gordon,A | Acquired via trade | Tex | | After |
| 4/2/2016 | Pit | Lawrie | Traded away | Oak | | After |
| 4/2/2016 | Pit | Lawrie | Acquired via trade | Oak | | After |
| 4/2/2016 | Pit | Lawrie | Acquired via trade | Oak | | After |
| 4/2/2016 | Pit | Reyes,Al | Traded away | Tex | | After |
| 4/2/2016 | Pit | Wieters | Traded away | Oak | | After |
| 4/2/2016 | Pit | Wieters | Traded away | Oak | | After |
| 4/2/2016 | Pit | Wieters | Acquired via trade | Tex | | After |
| 4/2/2016 | Pit | Wieters | Acquired via trade | Oak | | After |
| 4/2/2016 | Pit | Winker | Traded away | Tex | | After |
| 4/10/2016 | ChN | Happ | Promoted to active roster | | | Before |
| 4/10/2016 | ChN | Ruf | Demoted to reserve roster | | | Before |
| 4/10/2016 | Pit | Nicasio | Promoted to active roster | | | Before |
| 4/10/2016 | Pit | Parker,B | Demoted to reserve roster | | | Before |
| 4/11/2016 | ChN | Fiers | Promoted to active roster | | | Before |
| 4/11/2016 | ChN | Thornton,M | Demoted to reserve roster | | | Before |
| 4/11/2016 | Cin | Ackley | Promoted to active roster | | | Before |
| 4/11/2016 | Cin | Shields | Demoted to reserve roster | | | Before |
| 4/11/2016 | Mil | Ramos,C | Promoted to active roster | | | Before |
| 4/11/2016 | Mil | Rosscup | Demoted to reserve roster | | | Before |
| 4/11/2016 | SD | Bonifacio,J | Promoted to active roster | | | Before |
| 4/11/2016 | SD | Schwarber | Demoted to reserve roster | | | Before |
| 4/12/2016 | Cin | Buchholz | Acquired via trade | Tex | | After |
| 4/12/2016 | Cin | de Aza | Traded away | SD | | After |
| 4/12/2016 | Cin | Ege | Acquired via trade | Bos | | After |
| 4/12/2016 | Cin | McHugh | Acquired via trade | Bos | | After |
| 4/12/2016 | Cin | Mills,A | Traded away | Bos | | After |
| 4/12/2016 | Cin | Reddick | Acquired via trade | SD | | After |
| 4/12/2016 | Cin | Shields | Traded away | SD | | After |
| 4/12/2016 | Cin | Smith,Mal | Traded away | Bos | | After |
| 4/12/2016 | Pit | Uribe | Acquired via trade | Bos | | After |
| 4/12/2016 | SD | de Aza | Acquired via trade | Cin | | After |
| 4/12/2016 | SD | Jones,A | Acquired via trade | Hou | | After |
| 4/12/2016 | SD | Reddick | Traded away | Cin | | After |
| 4/12/2016 | SD | Rodriguez,Al | Acquired via trade | Bos | | After |
| 4/12/2016 | SD | Shields | Acquired via trade | Cin | | After |
| 4/12/2016 | StL | Freese | Acquired via trade | Bos | | After |
| 4/13/2016 | Ari | Schultz,J | Signed | | | Before |
| 4/13/2016 | Atl | Garcia,Le | Signed | | | Before |
| 4/13/2016 | Atl | Harang | Released | | | Before |
| 4/13/2016 | Atl | Morgan | Released | | | Before |
| 4/13/2016 | Atl | Revere | Demoted to reserve roster | | | Before |
| 4/13/2016 | Atl | Schafer,J | Released | | | Before |
| 4/13/2016 | Atl | Wallace | Signed | | | Before |
| 4/13/2016 | Atl | Wallace | Promoted to active roster | | | Before |
| 4/13/2016 | ChN | Fiers | Demoted to reserve roster | | | Before |
| 4/13/2016 | ChN | Fiers | Promoted to active roster | | | Before |
| 4/13/2016 | ChN | Layne | Signed | | | Before |
| 4/13/2016 | ChN | Layne | Promoted to active roster | | | Before |
| 4/13/2016 | ChN | Liriano,R | Signed | | | Before |
| 4/13/2016 | ChN | Mercer | Released | | | Before |
| 4/13/2016 | ChN | Ohlendorf | Released | | | Before |
| 4/13/2016 | ChN | Parra,M | Demoted to reserve roster | | | Before |
| 4/13/2016 | ChN | Powell | Demoted to reserve roster | | | Before |
| 4/13/2016 | ChN | Ruf | Released | | | Before |
| 4/13/2016 | ChN | Sands | Signed | | | Before |
| 4/13/2016 | ChN | Sands | Promoted to active roster | | | Before |
| 4/13/2016 | ChN | Thornton,M | Released | | | Before |
| 4/13/2016 | ChN | Webb,R | Signed | | | Before |
| 4/13/2016 | Cin | Ackley | Demoted to reserve roster | | | Before |
| 4/13/2016 | Cin | Buchholz | Promoted to active roster | | | Before |
| 4/13/2016 | Cin | Colon,C | Demoted to reserve roster | | | Before |
| 4/13/2016 | Cin | Hammel | Demoted to reserve roster | | | Before |
| 4/13/2016 | Cin | Howell | Released | | | Before |
| 4/13/2016 | Cin | Mercer | Released | | | Before |
| 4/13/2016 | Cin | Mercer | Signed | | | Before |
| 4/13/2016 | Cin | Moss | Demoted to reserve roster | | | Before |
| 4/13/2016 | Cin | Reed,Ad | Demoted to reserve roster | | | Before |
| 4/13/2016 | Cin | Walters,Z | Signed | | | Before |
| 4/13/2016 | Cin | Walters,Z | Promoted to active roster | | | Before |
| 4/13/2016 | Cin | Givens | Acquired via trade | Tex | | After |
| 4/13/2016 | Col | Beckham,T | Released | | | Before |
| 4/13/2016 | Col | Casali | Released | | | Before |
| 4/13/2016 | LAN | Chargois | Demoted to reserve roster | | | Before |
| 4/13/2016 | LAN | Gentry | Signed | | | Before |
| 4/13/2016 | LAN | Gentry | Promoted to active roster | | | Before |
| 4/13/2016 | LAN | Lohse | Released | | | Before |
| 4/13/2016 | Phi | Murphy,Dav | Released | | | Before |
| 4/13/2016 | Phi | Neris | Signed | | | Before |
| 4/13/2016 | SD | Bonifacio,J | Demoted to reserve roster | | | Before |
| 4/13/2016 | SD | de Aza | Promoted to active roster | | | Before |
| 4/13/2016 | SD | Fulmer | Demoted to reserve roster | | | Before |
| 4/13/2016 | SD | Gindl | Demoted to reserve roster | | | Before |
| 4/13/2016 | SD | Shields | Promoted to active roster | | | Before |
| 4/13/2016 | SF | Oberholtzer | Released | | | Before |
| 4/13/2016 | SF | Okert | Signed | | | Before |
| 4/13/2016 | StL | Thayer | Signed | | | Before |
| 4/18/2016 | Ari | McCann,J | Demoted to reserve roster | | | Before |
| 4/18/2016 | ChN | Campos,L | Released | | | Before |
| 4/18/2016 | ChN | Campos,L | Signed | | | Before |
| 4/18/2016 | ChN | Campos,L | Signed | | | Before |
| 4/18/2016 | ChN | Quackenbush | Demoted to reserve roster | | | Before |
| 4/18/2016 | ChN | Webb,R | Promoted to active roster | | | Before |
| 4/18/2016 | NYN | Blackmon | Demoted to reserve roster | | | Before |
| 4/18/2016 | NYN | Fisher,D | Promoted to active roster | | | Before |
| 4/18/2016 | Phi | Gomes,B | Promoted to active roster | | | Before |
| 4/18/2016 | Phi | Perkins | Demoted to reserve roster | | | Before |
| 4/18/2016 | SF | Alvarez,P | Promoted to active roster | | | Before |
| 4/18/2016 | SF | Heston | Promoted to active roster | | | Before |
| 4/18/2016 | SF | McCarthy | Demoted to reserve roster | | | Before |
| 4/18/2016 | SF | Okert | Promoted to active roster | | | Before |
| 4/18/2016 | SF | Sandoval | Demoted to reserve roster | | | Before |
| 4/19/2016 | Col | Brach | Traded away | SD | | After |
| 4/19/2016 | Col | Fulmer | Acquired via trade | SD | | After |
| 4/19/2016 | Pit | Nicasio | Traded away | NYA | | After |
| 4/19/2016 | Pit | Rondon,Jos | Acquired via trade | NYA | | After |
| 4/19/2016 | SD | Brach | Acquired via trade | Col | | After |
| 4/19/2016 | SD | Fulmer | Traded away | Col | | After |
| 4/20/2016 | Ari | Straily | Signed | | | Before |
| 4/20/2016 | ChN | Liriano,R | Released | | | Before |
| 4/20/2016 | ChN | Parra,M | Released | | | Before |
| 4/20/2016 | ChN | Rosales | Signed | | | Before |
| 4/20/2016 | Cin | Iglesias,R | Demoted to reserve roster | | | Before |
| 4/20/2016 | Cin | Moss | Promoted to active roster | | | Before |
| 4/20/2016 | LAN | Devenski | Signed | | | Before |
| 4/20/2016 | Mil | Holdzkom | Released | | | Before |
| 4/20/2016 | Mil | Mujica | Signed | | | Before |
| 4/20/2016 | Mil | Parker,Jarre | Released | | | Before |
| 4/20/2016 | Mil | Rosscup | Released | | | Before |
| 4/20/2016 | Mil | Walters,J | Signed | | | Before |
| 4/20/2016 | Mil | Wright,S | Signed | | | Before |
| 4/20/2016 | Phi | Morgan | Signed | | | Before |
| 4/20/2016 | Pit | Diaz,El | Demoted to reserve roster | | | Before |
| 4/20/2016 | Pit | Mercer | Signed | | | Before |
| 4/20/2016 | Pit | Parker,B | Released | | | Before |
| 4/20/2016 | Pit | Phelps,D | Promoted to active roster | | | Before |
| 4/20/2016 | Pit | Walsh | Released | | | Before |
| 4/20/2016 | SD | Spangenberg | Demoted to reserve roster | | | Before |
| 4/20/2016 | StL | Freese | Demoted to reserve roster | | | Before |
| 4/20/2016 | StL | Thayer | Promoted to active roster | | | Before |
| 4/20/2016 | StL | Tilson | Demoted to reserve roster | | | Before |
| 4/22/2016 | LAN | Colabello | Demoted to reserve roster | | | Before |
| 4/25/2016 | Ari | Jagielo | Promoted to active roster | | | Before |
| 4/25/2016 | Ari | Lindgren | Demoted to reserve roster | | | Before |
| 4/25/2016 | Ari | Straily | Promoted to active roster | | | Before |
| 4/25/2016 | ChN | Campos,L | Demoted to reserve roster | | | Before |
| 4/25/2016 | ChN | Campos,L | Promoted to active roster | | | Before |
| 4/25/2016 | ChN | Fiers | Demoted to reserve roster | | | Before |
| 4/25/2016 | ChN | Fiers | Promoted to active roster | | | Before |
| 4/25/2016 | ChN | Motter | Demoted to reserve roster | | | Before |
| 4/25/2016 | ChN | Rosales | Promoted to active roster | | | Before |
| 4/25/2016 | Cin | Garcia,Y | Demoted to reserve roster | | | Before |
| 4/25/2016 | Cin | Iglesias,R | Promoted to active roster | | | Before |
| 4/25/2016 | Col | Castro,M | Demoted to reserve roster | | | Before |
| 4/25/2016 | Col | Fulmer | Promoted to active roster | | | Before |
| 4/25/2016 | Col | Hanson | Promoted to active roster | | | Before |
| 4/25/2016 | Col | Overton | Promoted to active roster | | | Before |
| 4/25/2016 | Col | Reed,Co | Demoted to reserve roster | | | Before |
| 4/25/2016 | Mia | Bradley,A | Demoted to reserve roster | | | Before |
| 4/25/2016 | Mia | Bradley,A | Promoted to active roster | | | Before |
| 4/25/2016 | Mia | Eovaldi | Demoted to reserve roster | | | Before |
| 4/25/2016 | Mia | Flaherty | Promoted to active roster | | | Before |
| 4/25/2016 | Mia | Santana,Da | Demoted to reserve roster | | | Before |
| 4/25/2016 | Mia | Santana,E | Demoted to reserve roster | | | Before |
| 4/25/2016 | Mia | Stites | Promoted to active roster | | | Before |
| 4/28/2016 | Cin | Davis,Ro | Traded away | Tex | | After |
| 4/28/2016 | Cin | Garcia,Y | Traded away | SD | | After |
| 4/28/2016 | Cin | Gonzalez,G | Acquired via trade | Mia | | After |
| 4/28/2016 | Cin | Hamilton,B | Acquired via trade | SD | | After |
| 4/28/2016 | Cin | Iglesias,R | Traded away | Mia | | After |
| 4/28/2016 | Cin | Murphy,Dan | Traded away | Det | | After |
| 4/28/2016 | Cin | Strop | Acquired via trade | Det | | After |
| 4/28/2016 | Mia | Gomes,Y | Acquired via trade | Det | | After |
| 4/28/2016 | Mia | Gonzalez,Ad | Traded away | NYA | | After |
| 4/28/2016 | Mia | Gonzalez,G | Traded away | Cin | | After |
| 4/28/2016 | Mia | Iglesias,R | Acquired via trade | Cin | | After |
| 4/28/2016 | Mia | Perez,S | Traded away | Det | | After |
| 4/28/2016 | Mia | Shaw,B | Acquired via trade | NYA | | After |
| 4/28/2016 | Mia | Volquez | Traded away | Det | | After |
| 4/28/2016 | Mia | Wood,A | Acquired via trade | Det | | After |
| 4/28/2016 | Phi | Adames,C | Acquired via trade | Hou | | After |
| 4/28/2016 | Phi | Cecchini,Gav | Traded away | Hou | | After |
| 4/28/2016 | Phi | Madson | Acquired via trade | Hou | | After |
| 4/28/2016 | Phi | Neris | Traded away | Hou | | After |
| 4/28/2016 | Phi | Sipp | Acquired via trade | Hou | | After |
| 4/28/2016 | Phi | Tomlin | Traded away | Hou | | After |
| 4/28/2016 | Phi | Wainwright | Acquired via trade | Hou | | After |
| 4/28/2016 | Phi | Zimmermann | Traded away | Hou | | After |
| 4/28/2016 | SD | Garcia,Y | Acquired via trade | Cin | | After |
| 4/28/2016 | SD | Hamilton,B | Traded away | Cin | | After |
| 4/29/2016 | ChN | Campos,L | Promoted to active roster | | | Before |
| 4/29/2016 | ChN | Keuchel | Demoted to reserve roster | | | Before |
| 4/29/2016 | ChN | Maness | Demoted to reserve roster | | | Before |
| 4/29/2016 | ChN | Powell | Promoted to active roster | | | Before |
| 4/29/2016 | Cin | Bedrosian | Signed | | | Before |
| 4/29/2016 | Cin | Drake | Signed | | | Before |
| 4/29/2016 | Cin | Drake | Promoted to active roster | | | Before |
| 4/29/2016 | Cin | Franklin | Released | | | Before |
| 4/29/2016 | Cin | Guerrero,A | Released | | | Before |
| 4/29/2016 | Cin | Harris,W | Demoted to reserve roster | | | Before |
| 4/29/2016 | Cin | Ramirez,Har | Demoted to reserve roster | | | Before |
| 4/29/2016 | Mil | Dickey | Signed | | | Before |
| 4/29/2016 | Mil | Roe | Released | | | Before |
| 4/29/2016 | Phi | Machi | Demoted to reserve roster | | | Before |
| 4/29/2016 | Phi | Morin,M | Demoted to reserve roster | | | Before |
| 4/29/2016 | Pit | Cishek | Demoted to reserve roster | | | Before |
| 4/29/2016 | Pit | Matusz | Promoted to active roster | | | Before |
| 4/29/2016 | Pit | Stanek | Signed | | | Before |
| 4/29/2016 | SF | Capuano | Signed | | | Before |
| 4/29/2016 | SF | Capuano | Promoted to active roster | | | Before |
| 4/29/2016 | SF | Dyson,J | Promoted to active roster | | | Before |
| 4/29/2016 | SF | Goeddel,T | Demoted to reserve roster | | | Before |
| 4/29/2016 | SF | Goeddel,T | Promoted to active roster | | | Before |
| 4/29/2016 | SF | Mazzaro | Signed | | | Before |
| 4/29/2016 | SF | Mazzaro | Demoted to reserve roster | | | Before |
| 4/29/2016 | SF | Mazzaro | Promoted to active roster | | | Before |
| 4/29/2016 | SF | Medlen | Demoted to reserve roster | | | Before |
| 4/29/2016 | SF | Okert | Released | | | Before |
| 4/29/2016 | SF | Valencia | Demoted to reserve roster | | | Before |
| 4/29/2016 | Was | Johnson,M | Released | | | Before |
| 5/1/2016 | Cin | Bethancourt | Promoted to active roster | | | Before |
| 5/1/2016 | Cin | Ege | Demoted to reserve roster | | | Before |
| 5/1/2016 | Cin | Hardy,B | Promoted to active roster | | | Before |
| 5/1/2016 | Cin | Montero,M | Demoted to reserve roster | | | Before |
| 5/2/2016 | Cin | Hamilton,B | Demoted to reserve roster | | | Before |
| 5/2/2016 | Cin | Ramirez,Har | Promoted to active roster | | | Before |
| 5/2/2016 | Mil | Alonso | Promoted to active roster | | | Before |
| 5/2/2016 | Mil | Dickey | Promoted to active roster | | | Before |
| 5/2/2016 | Mil | Gordon,D | Demoted to reserve roster | | | Before |
| 5/2/2016 | Mil | Kelly,J | Demoted to reserve roster | | | Before |
| 5/2/2016 | NYN | Blackmon | Promoted to active roster | | | Before |
| 5/2/2016 | NYN | Hinojosa | Demoted to reserve roster | | | Before |
| 5/2/2016 | Pit | Cishek | Promoted to active roster | | | Before |
| 5/2/2016 | Pit | Colon,B | Demoted to reserve roster | | | Before |
| 5/2/2016 | SD | d'Arnaud,T | Demoted to reserve roster | | | Before |
| 5/6/2016 | Ari | Jagielo | Demoted to reserve roster | | | Before |
| 5/6/2016 | Ari | McCann,J | Promoted to active roster | | | Before |
| 5/6/2016 | Ari | Straily | Demoted to reserve roster | | | Before |
| 5/6/2016 | Ari | Zimmer,K | Promoted to active roster | | | Before |
| 5/6/2016 | ChN | Andriese | Demoted to reserve roster | | | Before |
| 5/6/2016 | ChN | Fiers | Demoted to reserve roster | | | Before |
| 5/6/2016 | ChN | Frazier,A | Signed | | | Before |
| 5/6/2016 | ChN | Keuchel | Promoted to active roster | | | Before |
| 5/6/2016 | ChN | Maness | Promoted to active roster | | | Before |
| 5/6/2016 | ChN | Motter | Released | | | Before |
| 5/6/2016 | Cin | Bedrosian | Released | | | Before |
| 5/6/2016 | Cin | Valdespin | Signed | | | Before |
| 5/6/2016 | Pit | Beltran | Demoted to reserve roster | | | Before |
| 5/6/2016 | Pit | Leon,S | Signed | | | Before |
| 5/9/2016 | ChN | Andriese | Promoted to active roster | | | Before |
| 5/9/2016 | ChN | Campbell | Demoted to reserve roster | | | Before |
| 5/9/2016 | ChN | Chen,W | Demoted to reserve roster | | | Before |
| 5/9/2016 | ChN | Fiers | Promoted to active roster | | | Before |
| 5/9/2016 | ChN | Happ | Demoted to reserve roster | | | Before |
| 5/9/2016 | ChN | Kang,J | Promoted to active roster | | | Before |
| 5/9/2016 | Cin | Colon,C | Promoted to active roster | | | Before |
| 5/9/2016 | Cin | Escobar,Edu | Demoted to reserve roster | | | Before |
| 5/9/2016 | Col | Mesoraco | Demoted to reserve roster | | | Before |
| 5/9/2016 | Col | Musgrove | Promoted to active roster | | | Before |
| 5/9/2016 | LAN | Chargois | Promoted to active roster | | | Before |
| 5/9/2016 | LAN | Devenski | Promoted to active roster | | | Before |
| 5/9/2016 | LAN | Gentry | Demoted to reserve roster | | | Before |
| 5/9/2016 | LAN | Zych | Demoted to reserve roster | | | Before |
| 5/9/2016 | Mil | Hundley | Demoted to reserve roster | | | Before |
| 5/9/2016 | Mil | Norris,De | Demoted to reserve roster | | | Before |
| 5/9/2016 | Mil | Norris,De | Promoted to active roster | | | Before |
| 5/9/2016 | Pit | Beltran | Promoted to active roster | | | Before |
| 5/9/2016 | Pit | Colon,B | Promoted to active roster | | | Before |
| 5/9/2016 | Pit | Leon,S | Demoted to reserve roster | | | Before |
| 5/9/2016 | Pit | Phelps,D | Demoted to reserve roster | | | Before |
| 5/9/2016 | SD | Bonifacio,J | Promoted to active roster | | | Before |
| 5/9/2016 | SD | Gindl | Promoted to active roster | | | Before |
| 5/9/2016 | SD | Martin,Co | Promoted to active roster | | | Before |
| 5/9/2016 | SD | Owens,R | Demoted to reserve roster | | | Before |
| 5/9/2016 | SD | Rodriguez,Al | Demoted to reserve roster | | | Before |
| 5/10/2016 | Was | Bassitt | Demoted to reserve roster | | | Before |
| 5/13/2016 | Ari | Bedrosian | Signed | | | Before |
| 5/13/2016 | Ari | Bedrosian | Promoted to active roster | | | Before |
| 5/13/2016 | Ari | Coulter | Demoted to reserve roster | | | Before |
| 5/13/2016 | ChN | Chen,W | Promoted to active roster | | | Before |
| 5/13/2016 | ChN | Fiers | Demoted to reserve roster | | | Before |
| 5/13/2016 | ChN | Happ | Promoted to active roster | | | Before |
| 5/13/2016 | ChN | Keuchel | Demoted to reserve roster | | | Before |
| 5/13/2016 | LAN | Aplin | Signed | | | Before |
| 5/13/2016 | LAN | Aplin | Promoted to active roster | | | Before |
| 5/13/2016 | LAN | Gentry | Released | | | Before |
| 5/13/2016 | Mil | Johnson,K | Released | | | Before |
| 5/13/2016 | Mil | Sanchez,T | Signed | | | Before |
| 5/13/2016 | Pit | Borenstein | Released | | | Before |
| 5/13/2016 | SD | Musgrave | Signed | | | Before |
| 5/13/2016 | SD | Owens,R | Released | | | Before |
| 5/13/2016 | SD | Rodriguez,S | Signed | | | Before |
| 5/16/2016 | Ari | Chapman,A | Promoted to active roster | | | Before |
| 5/16/2016 | Ari | Holland,D | Demoted to reserve roster | | | Before |
| 5/16/2016 | Ari | Straily | Promoted to active roster | | | Before |
| 5/16/2016 | Ari | Zimmer,K | Demoted to reserve roster | | | Before |
| 5/16/2016 | ChN | Andriese | Demoted to reserve roster | | | Before |
| 5/16/2016 | ChN | Campbell | Promoted to active roster | | | Before |
| 5/16/2016 | ChN | Hendriks | Demoted to reserve roster | | | Before |
| 5/16/2016 | ChN | Keuchel | Promoted to active roster | | | Before |
| 5/16/2016 | ChN | Liberatore | Promoted to active roster | | | Before |
| 5/16/2016 | ChN | Maness | Demoted to reserve roster | | | Before |
| 5/16/2016 | ChN | Quackenbush | Promoted to active roster | | | Before |
| 5/16/2016 | ChN | Simmons,A | Demoted to reserve roster | | | Before |
| 5/16/2016 | Cin | Bethancourt | Demoted to reserve roster | | | Before |
| 5/16/2016 | Cin | Montero,M | Promoted to active roster | | | Before |
| 5/16/2016 | Col | Griffin | Demoted to reserve roster | | | Before |
| 5/16/2016 | Col | Reed,Co | Promoted to active roster | | | Before |
| 5/16/2016 | Col | Reed,M | Released | | | Before |
| 5/16/2016 | Col | Scavuzzo | Signed | | | Before |
| 5/16/2016 | Mia | Eovaldi | Promoted to active roster | | | Before |
| 5/16/2016 | Mia | Iglesias,R | Demoted to reserve roster | | | Before |
| 5/16/2016 | Mia | Santana,Da | Promoted to active roster | | | Before |
| 5/16/2016 | Mil | Forsythe | Demoted to reserve roster | | | Before |
| 5/16/2016 | Mil | Marrero,D | Promoted to active roster | | | Before |
| 5/16/2016 | Mil | Sanchez,T | Promoted to active roster | | | Before |
| 5/16/2016 | StL | Buchter | Signed | | | Before |
| 5/18/2016 | StL | Young,CR | Demoted to reserve roster | | | Before |
| 5/20/2016 | ChN | Maness | Released | | | Before |
| 5/20/2016 | Cin | Ramirez,Al | Signed | | | Before |
| 5/20/2016 | Cin | Valdespin | Released | | | Before |
| 5/20/2016 | Col | Davidson | Signed | | | Before |
| 5/20/2016 | Col | Kemmer | Released | | | Before |
| 5/20/2016 | Mil | Joyce | Signed | | | Before |
| 5/20/2016 | Mil | Partch | Released | | | Before |
| 5/20/2016 | Phi | Greene,S | Released | | | Before |
| 5/20/2016 | Phi | Pivetta | Signed | | | Before |
| 5/20/2016 | SF | Anderson,Chr | Released | | | Before |
| 5/20/2016 | SF | Gillaspie | Signed | | | Before |
| 5/20/2016 | SF | Motter | Signed | | | Before |
| 5/20/2016 | Was | Chavez | Promoted to active roster | | | Before |
| 5/20/2016 | Was | Goeddel,E | Promoted to active roster | | | Before |
| 5/20/2016 | Was | Kela | Demoted to reserve roster | | | Before |
| 5/23/2016 | Ari | Delmonico | Signed | | | Before |
| 5/23/2016 | Ari | Jagielo | Released | | | Before |
| 5/23/2016 | ChN | Andriese | Promoted to active roster | | | Before |
| 5/23/2016 | ChN | Guerra,Ju | Signed | | | Before |
| 5/23/2016 | ChN | Guerra,Ju | Promoted to active roster | | | Before |
| 5/23/2016 | ChN | Liberatore | Demoted to reserve roster | | | Before |
| 5/23/2016 | ChN | Quackenbush | Released | | | Before |
| 5/23/2016 | Cin | Ackley | Promoted to active roster | | | Before |
| 5/23/2016 | Cin | Loup | Signed | | | Before |
| 5/23/2016 | Cin | Quackenbush | Signed | | | Before |
| 5/23/2016 | Cin | Ramirez,Al | Released | | | Before |
| 5/23/2016 | Cin | Reddick | Demoted to reserve roster | | | Before |
| 5/23/2016 | Col | Castro,M | Promoted to active roster | | | Before |
| 5/23/2016 | Col | Santana,Do | Demoted to reserve roster | | | Before |
| 5/23/2016 | LAN | Bowman | Signed | | | Before |
| 5/23/2016 | LAN | Chargois | Demoted to reserve roster | | | Before |
| 5/23/2016 | LAN | Herrmann,C | Signed | | | Before |
| 5/23/2016 | LAN | Herrmann,C | Promoted to active roster | | | Before |
| 5/23/2016 | LAN | Parker,Jarro | Released | | | Before |
| 5/23/2016 | Mia | Bradley,A | Promoted to active roster | | | Before |
| 5/23/2016 | Mia | DeSclafani | Demoted to reserve roster | | | Before |
| 5/23/2016 | Mil | Dickey | Demoted to reserve roster | | | Before |
| 5/23/2016 | Mil | Kelly,J | Promoted to active roster | | | Before |
| 5/23/2016 | Phi | Morgan | Released | | | Before |
| 5/23/2016 | Phi | Vincent | Signed | | | Before |
| 5/23/2016 | StL | Buchter | Promoted to active roster | | | Before |
| 5/23/2016 | Was | Heathcott | Released | | | Before |
| 5/24/2016 | SF | Brown,T | Promoted to active roster | | | Before |
| 5/24/2016 | SF | Phegley | Demoted to reserve roster | | | Before |
| 5/25/2016 | LAN | Chargois | Promoted to active roster | | | Before |
| 5/25/2016 | LAN | Devenski | Demoted to reserve roster | | | Before |
| 5/26/2016 | Pit | Cabrera,Me | Acquired via trade | Det | | After |
| 5/26/2016 | Pit | Gordon,A | Traded away | KC | | After |
| 5/26/2016 | Pit | Gray,S | Acquired via trade | KC | | After |
| 5/26/2016 | Pit | Jankowski,T | Traded away | Det | | After |
| 5/26/2016 | Pit | Kazmir | Acquired via trade | KC | | After |
| 5/26/2016 | Pit | Phelps,D | Traded away | KC | | After |
| 5/26/2016 | Pit | Ruiz,R | Traded away | Det | | After |
| 5/26/2016 | Pit | Valera | Acquired via trade | KC | | After |
| 5/26/2016 | Pit | Wacha | Traded away | KC | | After |
| 5/26/2016 | Pit | Wong,K | Traded away | KC | | After |
| 5/26/2016 | Pit | Young,CB | Acquired via trade | Det | | After |
| 5/27/2016 | ChN | Andriese | Demoted to reserve roster | | | Before |
| 5/27/2016 | ChN | Liberatore | Promoted to active roster | | | Before |
| 5/27/2016 | ChN | Rosales | Released | | | Before |
| 5/27/2016 | ChN | Shaffer | Promoted to active roster | | | Before |
| 5/27/2016 | Mil | Mujica | Released | | | Before |
| 5/27/2016 | Pit | Colon,B | Demoted to reserve roster | | | Before |
| 5/27/2016 | Pit | Leon,S | Released | | | Before |
| 5/27/2016 | Pit | Wilson,Ja | Released | | | Before |
| 5/27/2016 | StL | Garcia,G | Released | | | Before |
| 5/27/2016 | Was | Morton | Released | | | Before |
| 5/27/2016 | Was | Perez,W | Signed | | | Before |
| 5/29/2016 | Mil | Holt,B | Demoted to reserve roster | | | Before |
| 5/30/2016 | Ari | Banuelos | Promoted to active roster | | | Before |
| 5/30/2016 | Ari | Coulter | Promoted to active roster | | | Before |
| 5/30/2016 | Ari | Duda | Demoted to reserve roster | | | Before |
| 5/30/2016 | Ari | Gonzalez,Ma | Promoted to active roster | | | Before |
| 5/30/2016 | Ari | Martin,L | Demoted to reserve roster | | | Before |
| 5/30/2016 | Ari | Pompey | Promoted to active roster | | | Before |
| 5/30/2016 | Ari | Sanchez,Gar | Demoted to reserve roster | | | Before |
| 5/30/2016 | Ari | Straily | Demoted to reserve roster | | | Before |
| 5/30/2016 | Atl | Miller,S | Demoted to reserve roster | | | Before |
| 5/30/2016 | Atl | Revere | Promoted to active roster | | | Before |
| 5/30/2016 | ChN | Andriese | Promoted to active roster | | | Before |
| 5/30/2016 | ChN | Campbell | Released | | | Before |
| 5/30/2016 | ChN | Fiers | Promoted to active roster | | | Before |
| 5/30/2016 | ChN | Happ | Demoted to reserve roster | | | Before |
| 5/30/2016 | Cin | Colon,C | Demoted to reserve roster | | | Before |
| 5/30/2016 | Cin | Escobar,Edu | Promoted to active roster | | | Before |
| 5/30/2016 | Mil | Joyce | Demoted to reserve roster | | | Before |
| 5/30/2016 | Mil | Joyce | Promoted to active roster | | | Before |
| 5/30/2016 | Mil | Walters,J | Promoted to active roster | | | Before |
| 5/30/2016 | NYN | Biagini | Promoted to active roster | | | Before |
| 5/30/2016 | NYN | de la Rosa,R | Demoted to reserve roster | | | Before |
| 5/30/2016 | Phi | Blanton | Demoted to reserve roster | | | Before |
| 5/30/2016 | Phi | Eibner | Signed | | | Before |
| 5/30/2016 | Phi | Hamilton,J | Released | | | Before |
| 5/30/2016 | Phi | Ramirez,E | Promoted to active roster | | | Before |
| 5/30/2016 | Pit | Aybar | Demoted to reserve roster | | | Before |
| 5/30/2016 | Pit | Caminero | Demoted to reserve roster | | | Before |
| 5/30/2016 | Pit | Colon,B | Promoted to active roster | | | Before |
| 5/30/2016 | Pit | Gray,S | Demoted to reserve roster | | | Before |
| 5/30/2016 | Pit | Mercer | Demoted to reserve roster | | | Before |
| 5/30/2016 | Pit | Mercer | Promoted to active roster | | | Before |
| 5/30/2016 | Pit | Motte | Promoted to active roster | | | Before |
| 5/30/2016 | Pit | Rondon,Jos | Demoted to reserve roster | | | Before |
| 5/30/2016 | Pit | Rondon,Jos | Promoted to active roster | | | Before |
| 5/30/2016 | Pit | Young,CB | Promoted to active roster | | | Before |
| 5/30/2016 | SF | Mazzaro | Demoted to reserve roster | | | Before |
| 5/30/2016 | SF | Valencia | Promoted to active roster | | | Before |
| 5/30/2016 | StL | Cain,M | Demoted to reserve roster | | | Before |
| 5/30/2016 | StL | Freese | Promoted to active roster | | | Before |
| 5/30/2016 | Was | Kiermaier | Demoted to reserve roster | | | Before |
| 5/30/2016 | Was | Martinez,J | Promoted to active roster | | | Before |
| 6/3/2016 | ChN | Campos,L | Released | | | Before |
| 6/3/2016 | ChN | Coleman,L | Signed | | | Before |
| 6/3/2016 | Cin | Ackley | Released | | | Before |
| 6/3/2016 | Cin | Garcia,G | Signed | | | Before |
| 6/3/2016 | Cin | Hamilton,B | Promoted to active roster | | | Before |
| 6/3/2016 | Cin | Quackenbush | Released | | | Before |
| 6/3/2016 | Cin | Rodriguez,N | Signed | | | Before |
| 6/3/2016 | Col | Davidson | Promoted to active roster | | | Before |
| 6/3/2016 | Col | Musgrove | Demoted to reserve roster | | | Before |
| 6/3/2016 | Col | Santana,Do | Promoted to active roster | | | Before |
| 6/3/2016 | Col | Travis,S | Demoted to reserve roster | | | Before |
| 6/3/2016 | Phi | Grossman | Signed | | | Before |
| 6/3/2016 | Phi | Morin,M | Released | | | Before |
| 6/3/2016 | Pit | Jackson,A | Demoted to reserve roster | | | Before |
| 6/3/2016 | Pit | Leon,S | Signed | | | Before |
| 6/3/2016 | Pit | Mercer | Promoted to active roster | | | Before |
| 6/3/2016 | Pit | Valera | Demoted to reserve roster | | | Before |
| 6/3/2016 | SF | Hunter,C | Signed | | | Before |
| 6/3/2016 | SF | Mazzaro | Released | | | Before |
| 6/3/2016 | SF | Medlen | Released | | | Before |
| 6/3/2016 | SF | Torres,A | Signed | | | Before |
| 6/3/2016 | StL | Jackson,E | Signed | | | Before |
| 6/3/2016 | Was | Brown,Aa | Released | | | Before |
| 6/3/2016 | Was | Lewis | Signed | | | Before |
| 6/6/2016 | Ari | Bedrosian | Demoted to reserve roster | | | Before |
| 6/6/2016 | Ari | Smith,W | Promoted to active roster | | | Before |
| 6/6/2016 | ChN | Coleman,L | Promoted to active roster | | | Before |
| 6/6/2016 | ChN | Happ | Promoted to active roster | | | Before |
| 6/6/2016 | ChN | Rea | Released | | | Before |
| 6/6/2016 | ChN | Webb,R | Demoted to reserve roster | | | Before |
| 6/6/2016 | Cin | Harris,W | Promoted to active roster | | | Before |
| 6/6/2016 | Cin | O'Day | Demoted to reserve roster | | | Before |
| 6/6/2016 | LAN | Blazek | Demoted to reserve roster | | | Before |
| 6/6/2016 | LAN | Bowman | Demoted to reserve roster | | | Before |
| 6/6/2016 | LAN | Bowman | Promoted to active roster | | | Before |
| 6/6/2016 | LAN | Matzek | Promoted to active roster | | | Before |
| 6/6/2016 | Mia | Santana,Da | Demoted to reserve roster | | | Before |
| 6/6/2016 | Mia | Santana,E | Promoted to active roster | | | Before |
| 6/6/2016 | Mia | Wood,A | Demoted to reserve roster | | | Before |
| 6/6/2016 | Pit | Leon,S | Released | | | Before |
| 6/6/2016 | Pit | Parker,Jarre | Signed | | | Before |
| 6/6/2016 | Pit | Stanek | Released | | | Before |
| 6/6/2016 | SD | Miley | Demoted to reserve roster | | | Before |
| 6/6/2016 | SD | Musgrave | Promoted to active roster | | | Before |
| 6/6/2016 | SD | Ranaudo | Demoted to reserve roster | | | Before |
| 6/6/2016 | SD | Ranaudo | Promoted to active roster | | | Before |
| 6/6/2016 | SD | Rodriguez,Al | Promoted to active roster | | | Before |
| 6/6/2016 | SD | Rodriguez,S | Promoted to active roster | | | Before |
| 6/6/2016 | SD | Sano | Demoted to reserve roster | | | Before |
| 6/6/2016 | SF | Brown,T | Demoted to reserve roster | | | Before |
| 6/6/2016 | SF | Capuano | Demoted to reserve roster | | | Before |
| 6/6/2016 | SF | Goeddel,T | Demoted to reserve roster | | | Before |
| 6/6/2016 | SF | Heston | Demoted to reserve roster | | | Before |
| 6/6/2016 | SF | Hunter,C | Promoted to active roster | | | Before |
| 6/6/2016 | SF | Phegley | Promoted to active roster | | | Before |
| 6/6/2016 | SF | Street | Promoted to active roster | | | Before |
| 6/6/2016 | SF | Torres,A | Promoted to active roster | | | Before |
| 6/6/2016 | StL | Freese | Demoted to reserve roster | | | Before |
| 6/6/2016 | StL | Young,CR | Promoted to active roster | | | Before |
| 6/9/2016 | Cin | Blash | Traded away | Was | | After |
| 6/9/2016 | Cin | Hammel | Traded away | Was | | After |
| 6/9/2016 | Cin | Perez,W | Acquired via trade | Was | | After |
| 6/9/2016 | Cin | Souza | Acquired via trade | Was | | After |
| 6/9/2016 | Was | Blash | Acquired via trade | Cin | | After |
| 6/9/2016 | Was | Hammel | Acquired via trade | Cin | | After |
| 6/9/2016 | Was | Perez,W | Traded away | Cin | | After |
| 6/9/2016 | Was | Souza | Traded away | Cin | | After |
| 6/10/2016 | ChN | Frazier,A | Promoted to active roster | | | Before |
| 6/10/2016 | ChN | Otero | Signed | | | Before |
| 6/10/2016 | ChN | Powell | Released | | | Before |
| 6/10/2016 | ChN | Webb,R | Released | | | Before |
| 6/10/2016 | Cin | Garcia,G | Released | | | Before |
| 6/10/2016 | Cin | Rodriguez,N | Released | | | Before |
| 6/10/2016 | Col | Kintzler | Signed | | | Before |
| 6/10/2016 | Phi | Parmelee | Signed | | | Before |
| 6/10/2016 | Phi | Pivetta | Released | | | Before |
| 6/12/2016 | Cin | Drury | Acquired via trade | ChA | | After |
| 6/12/2016 | Cin | Finnegan | Acquired via trade | ChA | | After |
| 6/12/2016 | Cin | Frazier,T | Traded away | ChA | | After |
| 6/12/2016 | Cin | Schebler | Acquired via trade | ChA | | After |
| 6/12/2016 | Phi | Vincent | Traded away | StL | | After |
| 6/12/2016 | Pit | Gray,S | Promoted to active roster | | | Before |
| 6/12/2016 | Pit | Mercer | Demoted to reserve roster | | | Before |
| 6/12/2016 | Pit | Rondon,Jos | Promoted to active roster | | | Before |
| 6/12/2016 | Pit | Valera | Demoted to reserve roster | | | Before |
| 6/12/2016 | Pit | Valera | Promoted to active roster | | | Before |
| 6/12/2016 | StL | Vincent | Acquired via trade | Phi | | After |
| 6/13/2016 | Ari | Coulter | Demoted to reserve roster | | | Before |
| 6/13/2016 | Ari | Gonzalez,Ma | Demoted to reserve roster | | | Before |
| 6/13/2016 | Ari | Martin,L | Promoted to active roster | | | Before |
| 6/13/2016 | Ari | Sanchez,Gar | Promoted to active roster | | | Before |
| 6/13/2016 | ChN | Guerra,Ju | Demoted to reserve roster | | | Before |
| 6/13/2016 | ChN | Otero | Promoted to active roster | | | Before |
| 6/13/2016 | Cin | Cuthbert | Promoted to active roster | | | Before |
| 6/13/2016 | Cin | Hamilton,B | Demoted to reserve roster | | | Before |
| 6/13/2016 | Cin | Hughes,P | Demoted to reserve roster | | | Before |
| 6/13/2016 | Cin | Loup | Released | | | Before |
| 6/13/2016 | Cin | Mahle | Released | | | Before |
| 6/13/2016 | Cin | Schebler | Demoted to reserve roster | | | Before |
| 6/13/2016 | Col | Barbato | Released | | | Before |
| 6/13/2016 | Col | Estevez | Signed | | | Before |
| 6/13/2016 | Col | Santana,Do | Demoted to reserve roster | | | Before |
| 6/13/2016 | LAN | Owings | Demoted to reserve roster | | | Before |
| 6/13/2016 | LAN | Peralta,Jh | Promoted to active roster | | | Before |
| 6/13/2016 | Mia | DeSclafani | Promoted to active roster | | | Before |
| 6/13/2016 | Mia | Santana,E | Demoted to reserve roster | | | Before |
| 6/13/2016 | Mil | Forsythe | Promoted to active roster | | | Before |
| 6/13/2016 | Mil | Hundley | Promoted to active roster | | | Before |
| 6/13/2016 | Mil | Sanchez,T | Demoted to reserve roster | | | Before |
| 6/13/2016 | Mil | Walters,J | Demoted to reserve roster | | | Before |
| 6/13/2016 | StL | Almonte,M | Demoted to reserve roster | | | Before |
| 6/13/2016 | StL | Almonte,M | Promoted to active roster | | | Before |
| 6/13/2016 | StL | Jackson,E | Released | | | Before |
| 6/13/2016 | StL | Knebel | Promoted to active roster | | | Before |
| 6/13/2016 | StL | Thayer | Demoted to reserve roster | | | Before |
| 6/13/2016 | StL | Velasquez | Demoted to reserve roster | | | Before |
| 6/13/2016 | StL | Vincent | Promoted to active roster | | | Before |
| 6/13/2016 | Was | Butler,B | Signed | | | Before |
| 6/13/2016 | Was | Butler,B | Promoted to active roster | | | Before |
| 6/13/2016 | Was | Teixeira | Demoted to reserve roster | | | Before |
| 6/16/2016 | Cin | Almora | Acquired via trade | NYA | | After |
| 6/16/2016 | Cin | Ellsbury | Traded away | NYA | | After |
| 6/16/2016 | Cin | Encarnacion | Traded away | NYA | | After |
| 6/16/2016 | Cin | Hosmer | Acquired via trade | NYA | | After |
| 6/16/2016 | Was | Law | Acquired via trade | Oak | | After |
| 6/17/2016 | Cin | Cuthbert | Demoted to reserve roster | | | Before |
| 6/17/2016 | Cin | Givens | Demoted to reserve roster | | | Before |
| 6/17/2016 | Cin | Kemp,T | Promoted to active roster | | | Before |
| 6/17/2016 | Cin | Reed,Ad | Promoted to active roster | | | Before |
| 6/17/2016 | Mia | Greene,S | Signed | | | Before |
| 6/17/2016 | Phi | Machi | Released | | | Before |
| 6/17/2016 | SF | Capuano | Released | | | Before |
| 6/17/2016 | SF | Suarez,A | Signed | | | Before |
| 6/20/2016 | ChN | Happ | Demoted to reserve roster | | | Before |
| 6/20/2016 | ChN | Liberatore | Demoted to reserve roster | | | Before |
| 6/20/2016 | ChN | Liberatore | Promoted to active roster | | | Before |
| 6/20/2016 | ChN | Montas | Promoted to active roster | | | Before |
| 6/20/2016 | ChN | Sands | Released | | | Before |
| 6/20/2016 | ChN | Simmons,A | Promoted to active roster | | | Before |
| 6/20/2016 | Cin | Colon,C | Promoted to active roster | | | Before |
| 6/20/2016 | Cin | Hamilton,B | Promoted to active roster | | | Before |
| 6/20/2016 | Cin | Kemp,T | Demoted to reserve roster | | | Before |
| 6/20/2016 | Cin | Martinez,JD | Demoted to reserve roster | | | Before |
| 6/20/2016 | Cin | Schebler | Promoted to active roster | | | Before |
| 6/20/2016 | Cin | Souza | Demoted to reserve roster | | | Before |
| 6/20/2016 | Col | Ervin | Promoted to active roster | | | Before |
| 6/20/2016 | Col | Estevez | Promoted to active roster | | | Before |
| 6/20/2016 | Col | Parra,G | Demoted to reserve roster | | | Before |
| 6/20/2016 | Col | Scavuzzo | Promoted to active roster | | | Before |
| 6/20/2016 | LAN | Bautista,J | Demoted to reserve roster | | | Before |
| 6/20/2016 | LAN | Bowman | Demoted to reserve roster | | | Before |
| 6/20/2016 | LAN | Bowman | Promoted to active roster | | | Before |
| 6/20/2016 | LAN | Devenski | Promoted to active roster | | | Before |
| 6/20/2016 | Mil | Dickey | Promoted to active roster | | | Before |
| 6/20/2016 | Mil | Manaea | Demoted to reserve roster | | | Before |
| 6/20/2016 | Mil | Smith,Joe | Demoted to reserve roster | | | Before |
| 6/20/2016 | Mil | Walters,J | Promoted to active roster | | | Before |
| 6/20/2016 | NYN | Scribner | Released | | | Before |
| 6/20/2016 | Phi | Carrera | Signed | | | Before |
| 6/20/2016 | Phi | Morin,M | Released | | | Before |
| 6/20/2016 | Phi | Morin,M | Signed | | | Before |
| 6/20/2016 | Phi | Parmelee | Released | | | Before |
| 6/20/2016 | Pit | Sabathia | Signed | | | Before |
| 6/20/2016 | Pit | Valera | Released | | | Before |
| 6/21/2016 | Pit | Aybar | Promoted to active roster | | | Before |
| 6/21/2016 | Pit | Rondon,Jos | Demoted to reserve roster | | | Before |
| 6/24/2016 | ChN | Chen,W | Demoted to reserve roster | | | Before |
| 6/24/2016 | ChN | Happ | Promoted to active roster | | | Before |
| 6/24/2016 | Cin | Buchholz | Demoted to reserve roster | | | Before |
| 6/24/2016 | Cin | Lyons | Promoted to active roster | | | Before |
| 6/26/2016 | Cin | Buchholz | Traded away | Phi | | After |
| 6/26/2016 | Cin | Colon,C | Traded away | StL | | After |
| 6/26/2016 | Cin | Michalczewski | Acquired via trade | Phi | | After |
| 6/26/2016 | Cin | Montero,M | Traded away | Phi | | After |
| 6/26/2016 | Cin | Peraza | Acquired via trade | StL | | After |
| 6/26/2016 | Cin | Refsnyder | Acquired via trade | StL | | After |
| 6/26/2016 | Cin | Walker,N | Traded away | StL | | After |
| 6/26/2016 | Mil | Rasmus,Col | Traded away | Phi | | After |
| 6/26/2016 | Mil | Shoemaker,M | Acquired via trade | Phi | | After |
| 6/26/2016 | Phi | Buchholz | Acquired via trade | Cin | | After |
| 6/26/2016 | Phi | Michalczewski | Traded away | Cin | | After |
| 6/26/2016 | Phi | Montero,M | Acquired via trade | Cin | | After |
| 6/26/2016 | Phi | Rasmus,Col | Acquired via trade | Mil | | After |
| 6/26/2016 | Phi | Shoemaker,M | Traded away | Mil | | After |
| 6/26/2016 | StL | Colon,C | Acquired via trade | Cin | | After |
| 6/26/2016 | StL | Peraza | Traded away | Cin | | After |
| 6/26/2016 | StL | Refsnyder | Traded away | Cin | | After |
| 6/26/2016 | StL | Walker,N | Acquired via trade | Cin | | After |
| 6/27/2016 | Ari | Delmonico | Released | | | Before |
| 6/27/2016 | Ari | Rojas | Released | | | Before |
| 6/27/2016 | ChN | Chen,W | Promoted to active roster | | | Before |
| 6/27/2016 | ChN | Coleman,L | Released | | | Before |
| 6/27/2016 | ChN | Guerra,Ju | Promoted to active roster | | | Before |
| 6/27/2016 | ChN | Hendriks | Promoted to active roster | | | Before |
| 6/27/2016 | ChN | Layne | Released | | | Before |
| 6/27/2016 | ChN | Montas | Demoted to reserve roster | | | Before |
| 6/27/2016 | Cin | Bethancourt | Promoted to active roster | | | Before |
| 6/27/2016 | Cin | Papi | Released | | | Before |
| 6/27/2016 | Col | Griffin | Promoted to active roster | | | Before |
| 6/27/2016 | Col | Weiss | Demoted to reserve roster | | | Before |
| 6/27/2016 | LAN | Blazek | Promoted to active roster | | | Before |
| 6/27/2016 | LAN | Putnam | Demoted to reserve roster | | | Before |
| 6/27/2016 | Mil | Fowler | Demoted to reserve roster | | | Before |
| 6/27/2016 | Mil | Joyce | Promoted to active roster | | | Before |
| 6/27/2016 | Mil | Kelly,J | Demoted to reserve roster | | | Before |
| 6/27/2016 | Mil | Taylor,T | Promoted to active roster | | | Before |
| 6/27/2016 | Phi | Blanton | Promoted to active roster | | | Before |
| 6/27/2016 | Phi | Buchholz | Promoted to active roster | | | Before |
| 6/27/2016 | Phi | Carpenter,DD | Demoted to reserve roster | | | Before |
| 6/27/2016 | Phi | Carpenter,DD | Promoted to active roster | | | Before |
| 6/27/2016 | Phi | Gomes,B | Demoted to reserve roster | | | Before |
| 6/27/2016 | Phi | Iannetta | Demoted to reserve roster | | | Before |
| 6/27/2016 | Phi | Iannetta | Promoted to active roster | | | Before |
| 6/27/2016 | Phi | Pearce | Demoted to reserve roster | | | Before |
| 6/27/2016 | Phi | Ruggiano | Demoted to reserve roster | | | Before |
| 6/27/2016 | Pit | Rzepczynski | Promoted to active roster | | | Before |
| 6/27/2016 | Pit | Young,CB | Demoted to reserve roster | | | Before |
| 6/27/2016 | StL | Almonte,M | Demoted to reserve roster | | | Before |
| 6/27/2016 | StL | Gyorko | Demoted to reserve roster | | | Before |
| 6/27/2016 | StL | Van Slyke | Promoted to active roster | | | Before |
| 6/27/2016 | StL | Velasquez | Promoted to active roster | | | Before |
| 6/30/2016 | Cin | Harris,W | Traded away | Det | | After |
| 7/1/2016 | Cin | Givens | Promoted to active roster | | | Before |
| 7/4/2016 | ChN | Brault | Promoted to active roster | | | Before |
| 7/4/2016 | ChN | Hernandez,E | Demoted to reserve roster | | | Before |
| 7/4/2016 | ChN | Rivera,T | Signed | | | Before |
| 7/4/2016 | ChN | Rivera,T | Promoted to active roster | | | Before |
| 7/4/2016 | ChN | Shaffer | Released | | | Before |
| 7/4/2016 | Cin | Cedeno | Demoted to reserve roster | | | Before |
| 7/4/2016 | Cin | Drury | Demoted to reserve roster | | | Before |
| 7/4/2016 | Cin | Ege | Promoted to active roster | | | Before |
| 7/4/2016 | Cin | Reddick | Promoted to active roster | | | Before |
| 7/4/2016 | Cin | Schebler | Demoted to reserve roster | | | Before |
| 7/4/2016 | Cin | Souza | Promoted to active roster | | | Before |
| 7/4/2016 | Col | Davidson | Released | | | Before |
| 7/4/2016 | Col | Kemmer | Signed | | | Before |
| 7/4/2016 | Mil | Hernandez,D | Demoted to reserve roster | | | Before |
| 7/4/2016 | Mil | Holt,B | Promoted to active roster | | | Before |
| 7/4/2016 | Mil | Joyce | Demoted to reserve roster | | | Before |
| 7/4/2016 | Mil | Smith,Joe | Promoted to active roster | | | Before |
| 7/4/2016 | Pit | Mercer | Promoted to active roster | | | Before |
| 7/4/2016 | Pit | Siegrist | Demoted to reserve roster | | | Before |
| 7/4/2016 | SF | Doolittle | Demoted to reserve roster | | | Before |
| 7/4/2016 | SF | McCarthy | Promoted to active roster | | | Before |
| 7/4/2016 | StL | Almonte,M | Promoted to active roster | | | Before |
| 7/4/2016 | StL | Vincent | Demoted to reserve roster | | | Before |
| 7/5/2016 | Phi | Coghlan | Demoted to reserve roster | | | Before |
| 7/5/2016 | Phi | Grossman | Promoted to active roster | | | Before |
| 7/7/2016 | Cin | Cedeno | Traded away | Was | | After |
| 7/7/2016 | Was | Cedeno | Acquired via trade | Cin | | After |
| 7/8/2016 | Cin | Almora | Demoted to reserve roster | | | Before |
| 7/8/2016 | Cin | Collmenter | Promoted to active roster | | | Before |
| 7/8/2016 | Cin | Kimbrel | Demoted to reserve roster | | | Before |
| 7/8/2016 | Cin | Moss | Demoted to reserve roster | | | Before |
| 7/8/2016 | Cin | Schebler | Promoted to active roster | | | Before |
| 7/8/2016 | SD | d'Arnaud,T | Promoted to active roster | | | Before |
| 7/8/2016 | SD | de Aza | Demoted to reserve roster | | | Before |
| 7/8/2016 | SD | Kelly,Cas | Demoted to reserve roster | | | Before |
| 7/8/2016 | SD | Sano | Promoted to active roster | | | Before |
| 7/8/2016 | SF | Quackenbush | Signed | | | Before |
| 7/8/2016 | Was | Barraclough | Demoted to reserve roster | | | Before |
| 7/8/2016 | Was | Cedeno | Promoted to active roster | | | Before |
| 7/8/2016 | Was | Goeddel,E | Demoted to reserve roster | | | Before |
| 7/8/2016 | Was | Hammel | Promoted to active roster | | | Before |
| 7/8/2016 | Was | Martinez,J | Demoted to reserve roster | | | Before |
| 7/8/2016 | Was | Teixeira | Promoted to active roster | | | Before |
| 7/9/2016 | Mia | Jay | Demoted to reserve roster | | | Before |
| 7/9/2016 | Mia | Kershaw | Demoted to reserve roster | | | Before |
| 7/9/2016 | Mia | Santana,E | Promoted to active roster | | | Before |
| 7/10/2016 | ChN | Almonte,A | Acquired via trade | ChA | | After |
| 7/15/2016 | Ari | Coulter | Released | | | Before |
| 7/15/2016 | Ari | Franklin | Signed | | | Before |
| 7/15/2016 | Cin | Coulter | Signed | | | Before |
| 7/15/2016 | Col | Rodon | Demoted to reserve roster | | | Before |
| 7/15/2016 | LAN | Chafin | Demoted to reserve roster | | | Before |
| 7/15/2016 | LAN | Ryu | Promoted to active roster | | | Before |
| 7/15/2016 | Mil | Joyce | Promoted to active roster | | | Before |
| 7/15/2016 | Mil | Zimmerman | Demoted to reserve roster | | | Before |
| 7/15/2016 | NYN | Henderson | Demoted to reserve roster | | | Before |
| 7/15/2016 | NYN | Hinojosa | Promoted to active roster | | | Before |
| 7/15/2016 | SF | Phegley | Demoted to reserve roster | | | Before |
| 7/15/2016 | SF | Quackenbush | Promoted to active roster | | | Before |
| 7/15/2016 | StL | Almonte,M | Released | | | Before |
| 7/15/2016 | StL | Gonzalez,S | Signed | | | Before |
| 7/15/2016 | StL | Gonzalez,S | Promoted to active roster | | | Before |
| 7/15/2016 | StL | Tilson | Promoted to active roster | | | Before |
| 7/15/2016 | StL | Walker,T | Demoted to reserve roster | | | Before |
| 7/15/2016 | Was | Martinez,J | Released | | | Before |
| 7/15/2016 | Was | Schimpf | Signed | | | Before |
| 7/17/2016 | Phi | Rupp | Acquired via trade | Cle | | After |
| 7/17/2016 | StL | Pillar | Traded away | Det | | After |
| 7/18/2016 | ChN | Almonte,A | Promoted to active roster | | | Before |
| 7/18/2016 | ChN | Happ | Demoted to reserve roster | | | Before |
| 7/18/2016 | Cin | Reinheimer | Released | | | Before |
| 7/18/2016 | Cin | Reinheimer | Signed | | | Before |
| 7/18/2016 | Mil | Dickey | Demoted to reserve roster | | | Before |
| 7/18/2016 | Mil | Manaea | Promoted to active roster | | | Before |
| 7/18/2016 | Phi | Carpenter,DD | Released | | | Before |
| 7/18/2016 | Phi | Davis,W | Demoted to reserve roster | | | Before |
| 7/18/2016 | Pit | Motte | Demoted to reserve roster | | | Before |
| 7/18/2016 | Pit | Siegrist | Promoted to active roster | | | Before |
| 7/18/2016 | Was | Baez,P | Demoted to reserve roster | | | Before |
| 7/18/2016 | Was | Butler,B | Demoted to reserve roster | | | Before |
| 7/18/2016 | Was | Kela | Promoted to active roster | | | Before |
| 7/18/2016 | Was | Kiermaier | Promoted to active roster | | | Before |
| 7/18/2016 | Was | Schimpf | Released | | | Before |
| 7/21/2016 | Phi | Bolsinger | Acquired via trade | Was | | After |
| 7/21/2016 | Phi | Eibner | Traded away | Was | | After |
| 7/21/2016 | Pit | Gray,S | Traded away | NYA | | After |
| 7/21/2016 | Pit | Grimm | Acquired via trade | Was | | After |
| 7/21/2016 | Pit | Tanaka | Acquired via trade | NYA | | After |
| 7/21/2016 | Was | Bolsinger | Traded away | Phi | | After |
| 7/21/2016 | Was | Eibner | Acquired via trade | Phi | | After |
| 7/21/2016 | Was | Grimm | Traded away | Pit | | After |
| 7/21/2016 | Was | Hendricks | Acquired via trade | Min | | After |
| 7/21/2016 | Was | Inciarte | Traded away | Min | | After |
| 7/21/2016 | Was | Law | Traded away | Oak | | After |
| 7/21/2016 | Was | Smoak | Acquired via trade | Oak | | After |
| 7/21/2016 | Was | Szczur | Acquired via trade | Oak | | After |
| 7/22/2016 | ChN | Almonte,A | Released | | | Before |
| 7/22/2016 | ChN | Fiers | Demoted to reserve roster | | | Before |
| 7/22/2016 | ChN | Happ | Promoted to active roster | | | Before |
| 7/22/2016 | ChN | Schimpf | Signed | | | Before |
| 7/22/2016 | ChN | Schimpf | Promoted to active roster | | | Before |
| 7/22/2016 | Cin | Coulter | Demoted to reserve roster | | | Before |
| 7/22/2016 | Cin | Cuthbert | Promoted to active roster | | | Before |
| 7/22/2016 | Col | Gose | Released | | | Before |
| 7/22/2016 | Phi | Coleman,L | Signed | | | Before |
| 7/22/2016 | Phi | Coleman,L | Promoted to active roster | | | Before |
| 7/22/2016 | Phi | Feldman | Signed | | | Before |
| 7/22/2016 | Phi | Rupp | Demoted to reserve roster | | | Before |
| 7/22/2016 | Phi | Thornton,Z | Released | | | Before |
| 7/22/2016 | Pit | Rzepczynski | Demoted to reserve roster | | | Before |
| 7/22/2016 | Was | Geltz | Released | | | Before |
| 7/22/2016 | Was | Taijeron | Signed | | | Before |
| 7/24/2016 | LAN | Bautista,J | Traded away | Pit | | After |
| 7/24/2016 | LAN | Niese | Traded away | Pit | | After |
| 7/24/2016 | LAN | Peralta,Jh | Traded away | Pit | | After |
| 7/24/2016 | LAN | Tanaka | Acquired via trade | Pit | | After |
| 7/24/2016 | Phi | Lowe | Acquired via trade | Was | | After |
| 7/24/2016 | Pit | Bautista,J | Acquired via trade | LAN | | After |
| 7/24/2016 | Pit | Colon,B | Traded away | KC | | After |
| 7/24/2016 | Pit | Kingham | Acquired via trade | KC | | After |
| 7/24/2016 | Pit | Niese | Acquired via trade | LAN | | After |
| 7/24/2016 | Pit | Peralta,Jh | Acquired via trade | LAN | | After |
| 7/24/2016 | Pit | Romo | Acquired via trade | Tex | | After |
| 7/24/2016 | Pit | Tanaka | Traded away | LAN | | After |
| 7/24/2016 | StL | Gregerson | Acquired via trade | NYA | | After |
| 7/24/2016 | StL | Lewis | Acquired via trade | Was | | After |
| 7/24/2016 | Was | Lewis | Traded away | StL | | After |
| 7/24/2016 | Was | Lowe | Traded away | Phi | | After |
| 7/25/2016 | Ari | Vizcaino | Demoted to reserve roster | | | Before |
| 7/25/2016 | ChN | Fiers | Promoted to active roster | | | Before |
| 7/25/2016 | ChN | Kennedy | Demoted to reserve roster | | | Before |
| 7/25/2016 | ChN | Kennedy | Promoted to active roster | | | Before |
| 7/25/2016 | ChN | Uehara | Demoted to reserve roster | | | Before |
| 7/25/2016 | Cin | Cuthbert | Demoted to reserve roster | | | Before |
| 7/25/2016 | Cin | Drury | Promoted to active roster | | | Before |
| 7/25/2016 | LAN | Bowman | Promoted to active roster | | | Before |
| 7/25/2016 | LAN | Colabello | Promoted to active roster | | | Before |
| 7/25/2016 | LAN | Herrmann,C | Demoted to reserve roster | | | Before |
| 7/25/2016 | LAN | Owings | Promoted to active roster | | | Before |
| 7/25/2016 | LAN | Ryu | Demoted to reserve roster | | | Before |
| 7/25/2016 | Mil | Fowler | Promoted to active roster | | | Before |
| 7/25/2016 | Mil | Joyce | Demoted to reserve roster | | | Before |
| 7/25/2016 | Pit | Caminero | Promoted to active roster | | | Before |
| 7/25/2016 | Pit | Niese | Demoted to reserve roster | | | Before |
| 7/25/2016 | Pit | Peralta,Jh | Demoted to reserve roster | | | Before |
| 7/25/2016 | Pit | Romo | Promoted to active roster | | | Before |
| 7/25/2016 | Was | Baez,P | Promoted to active roster | | | Before |
| 7/28/2016 | Cin | Howell | Acquired via trade | NYA | | After |
| 7/28/2016 | Cin | O'Day | Traded away | NYA | | After |
| 7/28/2016 | Cin | Plawecki | Acquired via trade | NYA | | After |
| 7/28/2016 | Cin | Strop | Traded away | NYA | | After |
| 7/28/2016 | LAN | Martin,Ru | Traded away | Phi | | After |
| 7/28/2016 | Phi | Martin,Ru | Acquired via trade | LAN | | After |
| 7/28/2016 | Pit | Cingrani | Traded away | Hou | | After |
| 7/28/2016 | Pit | Lincecum | Acquired via trade | Tex | | After |
| 7/28/2016 | Pit | Lind | Acquired via trade | Hou | | After |
| 7/28/2016 | Pit | Napoli | Traded away | Hou | | After |
| 7/29/2016 | Cin | Campos,L | Signed | | | Before |
| 7/29/2016 | Cin | Campos,L | Promoted to active roster | | | Before |
| 7/29/2016 | Cin | Collmenter | Demoted to reserve roster | | | Before |
| 7/29/2016 | Cin | Coulter | Released | | | Before |
| 7/29/2016 | Cin | Plawecki | Demoted to reserve roster | | | Before |
| 7/29/2016 | Col | Scavuzzo | Released | | | Before |
| 7/29/2016 | Phi | Feldman | Released | | | Before |
| 7/29/2016 | Pit | Sabathia | Released | | | Before |
| 7/29/2016 | SD | Hultzen | Released | | | Before |
| 7/30/2016 | Atl | Morrow | Demoted to reserve roster | | | Before |
| 7/31/2016 | ChN | Chen,W | Traded away | Det | | After |
| 7/31/2016 | ChN | Jankowski,T | Acquired via trade | Det | | After |
| 7/31/2016 | ChN | Uehara | Traded away | Det | | After |
| 7/31/2016 | Cin | Hosmer | Traded away | Oak | | After |
| 7/31/2016 | Cin | Swihart | Acquired via trade | Oak | | After |
| 7/31/2016 | Col | Bailey,H | Traded away | KC | | After |
| 7/31/2016 | Col | Cozart | Traded away | KC | | After |
| 7/31/2016 | Col | Estevez | Traded away | KC | | After |
| 7/31/2016 | Col | Gattis | Acquired via trade | KC | | After |
| 7/31/2016 | Col | Hardy,J | Acquired via trade | KC | | After |
| 7/31/2016 | Col | Sadzeck | Acquired via trade | Tex | | After |
| 7/31/2016 | Col | Winker | Acquired via trade | Tex | | After |
| 8/1/2016 | Ari | Bedrosian | Promoted to active roster | | | Before |
| 8/1/2016 | Ari | Difo | Promoted to active roster | | | Before |
| 8/1/2016 | Ari | Glasnow | Demoted to reserve roster | | | Before |
| 8/1/2016 | Ari | Moore,M | Promoted to active roster | | | Before |
| 8/1/2016 | Atl | Garcia,Le | Promoted to active roster | | | Before |
| 8/1/2016 | Atl | Garza | Promoted to active roster | | | Before |
| 8/1/2016 | Atl | Middlebrooks | Demoted to reserve roster | | | Before |
| 8/1/2016 | ChN | Jankowski,T | Promoted to active roster | | | Before |
| 8/1/2016 | ChN | Kennedy | Released | | | Before |
| 8/1/2016 | ChN | Norris,B | Signed | | | Before |
| 8/1/2016 | ChN | Norris,B | Promoted to active roster | | | Before |
| 8/1/2016 | ChN | Raburn | Released | | | Before |
| 8/1/2016 | ChN | Segedin | Signed | | | Before |
| 8/1/2016 | ChN | Segedin | Promoted to active roster | | | Before |
| 8/1/2016 | Cin | Almora | Promoted to active roster | | | Before |
| 8/1/2016 | Cin | Cuthbert | Promoted to active roster | | | Before |
| 8/1/2016 | Cin | Drury | Demoted to reserve roster | | | Before |
| 8/1/2016 | LAN | Hochevar | Demoted to reserve roster | | | Before |
| 8/1/2016 | Mil | Gordon,D | Promoted to active roster | | | Before |
| 8/1/2016 | Mil | Taylor,T | Demoted to reserve roster | | | Before |
| 8/1/2016 | Mil | Valbuena | Demoted to reserve roster | | | Before |
| 8/1/2016 | Mil | Zimmerman | Promoted to active roster | | | Before |
| 8/1/2016 | Phi | Blanton | Demoted to reserve roster | | | Before |
| 8/1/2016 | Phi | Grossman | Demoted to reserve roster | | | Before |
| 8/1/2016 | Phi | Iannetta | Demoted to reserve roster | | | Before |
| 8/1/2016 | Phi | Manship | Demoted to reserve roster | | | Before |
| 8/1/2016 | Phi | Pearce | Promoted to active roster | | | Before |
| 8/1/2016 | Phi | Ruggiano | Promoted to active roster | | | Before |
| 8/1/2016 | Phi | Utley | Demoted to reserve roster | | | Before |
| 8/1/2016 | Phi | Utley | Promoted to active roster | | | Before |
| 8/1/2016 | Pit | Bautista,J | Promoted to active roster | | | Before |
| 8/1/2016 | Pit | Lawrie | Demoted to reserve roster | | | Before |
| 8/1/2016 | Pit | Niese | Demoted to reserve roster | | | Before |
| 8/1/2016 | Pit | Niese | Promoted to active roster | | | Before |
| 8/1/2016 | StL | Gonzalez,S | Demoted to reserve roster | | | Before |
| 8/1/2016 | StL | Skaggs | Promoted to active roster | | | Before |
| 8/1/2016 | Was | Perez,H | Signed | | | Before |
| 8/1/2016 | Was | Shreve | Released | | | Before |
| 8/4/2016 | Phi | Eflin | Traded away | Was | | After |
| 8/4/2016 | Phi | Grossman | Traded away | Was | | After |
| 8/4/2016 | Phi | Kela | Acquired via trade | Was | | After |
| 8/4/2016 | Phi | Pearce | Traded away | Was | | After |
| 8/4/2016 | Phi | Ramos,A | Acquired via trade | Was | | After |
| 8/4/2016 | Was | Eflin | Acquired via trade | Phi | | After |
| 8/4/2016 | Was | Grossman | Acquired via trade | Phi | | After |
| 8/4/2016 | Was | Kela | Traded away | Phi | | After |
| 8/4/2016 | Was | Pearce | Acquired via trade | Phi | | After |
| 8/4/2016 | Was | Ramos,A | Traded away | Phi | | After |
| 8/5/2016 | NYN | Despaigne | Promoted to active roster | | | Before |
| 8/5/2016 | NYN | Nola,Aa | Demoted to reserve roster | | | Before |
| 8/5/2016 | Phi | Coleman,L | Demoted to reserve roster | | | Before |
| 8/5/2016 | Was | Ahmed | Demoted to reserve roster | | | Before |
| 8/5/2016 | Was | Barraclough | Promoted to active roster | | | Before |
| 8/5/2016 | Was | Goeddel,E | Promoted to active roster | | | Before |
| 8/5/2016 | Was | Pearce | Demoted to reserve roster | | | Before |
| 8/5/2016 | Was | Tejada | Promoted to active roster | | | Before |
| 8/7/2016 | Col | Gomez,J | Traded away | Was | | After |
| 8/7/2016 | Col | Gonzalez,Ch | Traded away | Oak | | After |
| 8/7/2016 | Was | Gomez,J | Acquired via trade | Col | | After |
| 8/8/2016 | ChN | Brault | Demoted to reserve roster | | | Before |
| 8/8/2016 | ChN | Brault | Promoted to active roster | | | Before |
| 8/8/2016 | ChN | Greene,C | Promoted to active roster | | | Before |
| 8/8/2016 | ChN | Hernandez,E | Promoted to active roster | | | Before |
| 8/8/2016 | ChN | Liberatore | Demoted to reserve roster | | | Before |
| 8/8/2016 | ChN | Norris,B | Demoted to reserve roster | | | Before |
| 8/8/2016 | ChN | Rivera,T | Demoted to reserve roster | | | Before |
| 8/8/2016 | Cin | Campos,L | Demoted to reserve roster | | | Before |
| 8/8/2016 | Cin | Collmenter | Promoted to active roster | | | Before |
| 8/8/2016 | Cin | Cuthbert | Demoted to reserve roster | | | Before |
| 8/8/2016 | Cin | Kimbrel | Promoted to active roster | | | Before |
| 8/8/2016 | Cin | Lyons | Demoted to reserve roster | | | Before |
| 8/8/2016 | Cin | Martinez,JD | Promoted to active roster | | | Before |
| 8/8/2016 | Cin | Moss | Promoted to active roster | | | Before |
| 8/8/2016 | Cin | Ramirez,Har | Demoted to reserve roster | | | Before |
| 8/8/2016 | Cin | Schebler | Demoted to reserve roster | | | Before |
| 8/8/2016 | Cin | Schebler | Promoted to active roster | | | Before |
| 8/8/2016 | Col | Hardy,J | Promoted to active roster | | | Before |
| 8/8/2016 | Col | Kintzler | Promoted to active roster | | | Before |
| 8/8/2016 | Col | Morneau | Promoted to active roster | | | Before |
| 8/8/2016 | Col | Musgrove | Promoted to active roster | | | Before |
| 8/8/2016 | Col | Rodon | Promoted to active roster | | | Before |
| 8/8/2016 | Col | Romano | Signed | | | Before |
| 8/8/2016 | Mil | Joyce | Promoted to active roster | | | Before |
| 8/8/2016 | Mil | Zimmerman | Demoted to reserve roster | | | Before |
| 8/8/2016 | Pit | Broxton,J | Demoted to reserve roster | | | Before |
| 8/8/2016 | Pit | Cishek | Demoted to reserve roster | | | Before |
| 8/8/2016 | Pit | Niese | Promoted to active roster | | | Before |
| 8/8/2016 | Pit | Peralta,Jh | Promoted to active roster | | | Before |
| 8/8/2016 | StL | Freese | Promoted to active roster | | | Before |
| 8/8/2016 | StL | Gregerson | Demoted to reserve roster | | | Before |
| 8/8/2016 | StL | Tilson | Demoted to reserve roster | | | Before |
| 8/8/2016 | StL | Upton,M | Demoted to reserve roster | | | Before |
| 8/8/2016 | StL | Upton,M | Promoted to active roster | | | Before |
| 8/8/2016 | StL | Walker,T | Promoted to active roster | | | Before |
| 8/11/2016 | Col | Parra,G | Traded away | Phi | | After |
| 8/11/2016 | Phi | Parra,G | Acquired via trade | Col | | After |
| 8/11/2016 | Phi | Werth | Acquired via trade | KC | | After |
| 8/12/2016 | Ari | Lindgren | Released | | | Before |
| 8/12/2016 | Cin | Almora | Demoted to reserve roster | | | Before |
| 8/12/2016 | Cin | Campos,L | Released | | | Before |
| 8/12/2016 | Cin | Drury | Promoted to active roster | | | Before |
| 8/12/2016 | Cin | Klein | Signed | | | Before |
| 8/12/2016 | LAN | Strahm | Signed | | | Before |
| 8/12/2016 | Phi | Carrera | Released | | | Before |
| 8/12/2016 | Phi | Gomes,B | Released | | | Before |
| 8/12/2016 | Phi | Sherfy | Signed | | | Before |
| 8/14/2016 | Cin | Bauers | Acquired via trade | Tor | | After |
| 8/14/2016 | Cin | Blackburn | Acquired via trade | Bos | | After |
| 8/14/2016 | Cin | Flores,W | Acquired via trade | Tor | | After |
| 8/14/2016 | Cin | Hoyt | Acquired via trade | Bos | | After |
| 8/14/2016 | Cin | Kemp,T | Traded away | Tor | | After |
| 8/14/2016 | Cin | Kimbrel | Traded away | Tor | | After |
| 8/14/2016 | Cin | Martinez,JD | Traded away | Tor | | After |
| 8/14/2016 | Cin | Myers | Acquired via trade | Tor | | After |
| 8/14/2016 | StL | Palka | Traded away | Was | | After |
| 8/14/2016 | StL | Santiago,H | Acquired via trade | Was | | After |
| 8/14/2016 | StL | Teixeira | Acquired via trade | Was | | After |
| 8/14/2016 | Was | Palka | Acquired via trade | StL | | After |
| 8/14/2016 | Was | Santiago,H | Traded away | StL | | After |
| 8/14/2016 | Was | Teixeira | Traded away | StL | | After |
| 8/15/2016 | Ari | Bedrosian | Demoted to reserve roster | | | Before |
| 8/15/2016 | Ari | Gonzalez,Ma | Promoted to active roster | | | Before |
| 8/15/2016 | ChN | Guerra,Ju | Demoted to reserve roster | | | Before |
| 8/15/2016 | ChN | Simmons,S | Promoted to active roster | | | Before |
| 8/15/2016 | Cin | Collmenter | Demoted to reserve roster | | | Before |
| 8/15/2016 | Cin | Drury | Demoted to reserve roster | | | Before |
| 8/15/2016 | Cin | Hoyt | Promoted to active roster | | | Before |
| 8/15/2016 | Cin | Schebler | Demoted to reserve roster | | | Before |
| 8/15/2016 | Col | Gonzalez,E | Signed | | | Before |
| 8/15/2016 | Phi | Manship | Promoted to active roster | | | Before |
| 8/15/2016 | Phi | Parra,G | Promoted to active roster | | | Before |
| 8/15/2016 | Phi | Ramos,A | Demoted to reserve roster | | | Before |
| 8/15/2016 | Phi | Rasmus,Col | Demoted to reserve roster | | | Before |
| 8/15/2016 | Phi | Ruggiano | Demoted to reserve roster | | | Before |
| 8/15/2016 | Pit | Bautista,J | Demoted to reserve roster | | | Before |
| 8/15/2016 | Pit | Broxton,J | Promoted to active roster | | | Before |
| 8/15/2016 | Pit | Diaz,El | Promoted to active roster | | | Before |
| 8/15/2016 | Pit | Holliday | Demoted to reserve roster | | | Before |
| 8/15/2016 | Pit | Parker,Jarre | Promoted to active roster | | | Before |
| 8/15/2016 | Pit | Reynolds,Mar | Demoted to reserve roster | | | Before |
| 8/15/2016 | Pit | Rondon,Jos | Promoted to active roster | | | Before |
| 8/15/2016 | Pit | Uribe | Demoted to reserve roster | | | Before |
| 8/15/2016 | SF | Cespedes | Demoted to reserve roster | | | Before |
| 8/15/2016 | SF | Goeddel,T | Promoted to active roster | | | Before |
| 8/15/2016 | SF | McCarthy | Demoted to reserve roster | | | Before |
| 8/15/2016 | SF | Motter | Promoted to active roster | | | Before |
| 8/15/2016 | StL | Adams,Ma | Demoted to reserve roster | | | Before |
| 8/15/2016 | StL | Cain,M | Released | | | Before |
| 8/15/2016 | StL | Freeman,S | Demoted to reserve roster | | | Before |
| 8/15/2016 | StL | Freeman,S | Promoted to active roster | | | Before |
| 8/15/2016 | StL | Santiago,H | Demoted to reserve roster | | | Before |
| 8/15/2016 | StL | Van Slyke | Demoted to reserve roster | | | Before |
| 8/15/2016 | StL | Vincent | Promoted to active roster | | | Before |
| 8/15/2016 | Was | Dull | Promoted to active roster | | | Before |
| 8/15/2016 | Was | Pearce | Promoted to active roster | | | Before |
| 8/15/2016 | Was | Smoak | Demoted to reserve roster | | | Before |
| 8/15/2016 | Was | Taijeron | Released | | | Before |
| 8/15/2016 | Was | Tejada | Released | | | Before |
| 8/15/2016 | Was | Villar | Promoted to active roster | | | Before |
| 8/16/2016 | Mia | Eovaldi | Demoted to reserve roster | | | Before |
| 8/18/2016 | Ari | Robles | Traded away | Oak | | After |
| 8/18/2016 | Ari | Smith,W | Traded away | KC | | After |
| 8/18/2016 | Cin | Ethier | Traded away | Pit | | After |
| 8/18/2016 | Mia | Gomes,Y | Demoted to reserve roster | | | Before |
| 8/18/2016 | Pit | Ethier | Acquired via trade | Cin | | After |
| 8/19/2016 | Ari | Boyd,M | Signed | | | Before |
| 8/19/2016 | LAN | Centeno | Signed | | | Before |
| 8/19/2016 | SF | Cain,M | Signed | | | Before |
| 8/19/2016 | SF | Motter | Released | | | Before |
| 8/21/2016 | Ari | Bracho | Traded away | Cin | | After |
| 8/21/2016 | Ari | Drury | Acquired via trade | Cin | | After |
| 8/21/2016 | Ari | Liriano,F | Traded away | Cin | | After |
| 8/21/2016 | Cin | Bracho | Acquired via trade | Ari | | After |
| 8/21/2016 | Cin | Drury | Traded away | Ari | | After |
| 8/21/2016 | Cin | Liriano,F | Acquired via trade | Ari | | After |
| 8/22/2016 | Ari | Boyd,M | Promoted to active roster | | | Before |
| 8/22/2016 | Ari | Drury | Demoted to reserve roster | | | Before |
| 8/22/2016 | Ari | Drury | Promoted to active roster | | | Before |
| 8/22/2016 | Ari | Norris,Da | Promoted to active roster | | | Before |
| 8/22/2016 | Ari | Straily | Promoted to active roster | | | Before |
| 8/22/2016 | Ari | Vizcaino | Promoted to active roster | | | Before |
| 8/22/2016 | ChN | Greene,C | Demoted to reserve roster | | | Before |
| 8/22/2016 | ChN | Kang,J | Demoted to reserve roster | | | Before |
| 8/22/2016 | ChN | Liberatore | Promoted to active roster | | | Before |
| 8/22/2016 | ChN | Norris,B | Promoted to active roster | | | Before |
| 8/22/2016 | Cin | Blackburn | Demoted to reserve roster | | | Before |
| 8/22/2016 | Cin | Hardy,B | Demoted to reserve roster | | | Before |
| 8/22/2016 | Cin | Perez,W | Released | | | Before |
| 8/22/2016 | Cin | Schebler | Promoted to active roster | | | Before |
| 8/22/2016 | Cin | Walters,Z | Released | | | Before |
| 8/22/2016 | Col | Canelo | Released | | | Before |
| 8/22/2016 | LAN | Aplin | Released | | | Before |
| 8/22/2016 | LAN | Centeno | Promoted to active roster | | | Before |
| 8/22/2016 | LAN | Matzek | Released | | | Before |
| 8/22/2016 | LAN | Strahm | Promoted to active roster | | | Before |
| 8/22/2016 | LAN | Zastryzny | Signed | | | Before |
| 8/22/2016 | LAN | Zastryzny | Promoted to active roster | | | Before |
| 8/22/2016 | Mil | Barnette,To | Promoted to active roster | | | Before |
| 8/22/2016 | Mil | Hernandez,D | Promoted to active roster | | | Before |
| 8/22/2016 | Mil | Joyce | Demoted to reserve roster | | | Before |
| 8/22/2016 | Mil | Smith,Joe | Demoted to reserve roster | | | Before |
| 8/22/2016 | Mil | Walters,J | Demoted to reserve roster | | | Before |
| 8/22/2016 | Mil | Zimmerman | Promoted to active roster | | | Before |
| 8/22/2016 | Phi | Cessa | Signed | | | Before |
| 8/22/2016 | Phi | Coghlan | Released | | | Before |
| 8/22/2016 | SF | Blanks | Released | | | Before |
| 8/22/2016 | SF | Cole,H | Promoted to active roster | | | Before |
| 8/22/2016 | SF | Heston | Released | | | Before |
| 8/22/2016 | SF | Mariot | Signed | | | Before |
| 8/22/2016 | SF | Mariot | Promoted to active roster | | | Before |
| 8/22/2016 | SF | Rondon,H | Demoted to reserve roster | | | Before |
| 8/22/2016 | SF | Sewald | Signed | | | Before |
| 8/22/2016 | SF | Sewald | Promoted to active roster | | | Before |
| 8/22/2016 | SF | Street | Demoted to reserve roster | | | Before |
| 8/22/2016 | StL | Gregerson | Promoted to active roster | | | Before |
| 8/22/2016 | StL | Young,CR | Demoted to reserve roster | | | Before |
| 8/26/2016 | Col | Weiss | Released | | | Before |
| 8/26/2016 | LAN | Blazek | Released | | | Before |
| 8/26/2016 | Pit | Broxton,J | Demoted to reserve roster | | | Before |
| 8/26/2016 | Pit | Cishek | Promoted to active roster | | | Before |
| 8/26/2016 | StL | Thayer | Released | | | Before |
| 8/27/2016 | Was | Matz | Demoted to reserve roster | | | Before |
| 8/27/2016 | Was | McCurry | Promoted to active roster | | | Before |
| 8/29/2016 | Ari | Banuelos | Demoted to reserve roster | | | Before |
| 8/29/2016 | Ari | Drury | Demoted to reserve roster | | | Before |
| 8/29/2016 | Ari | Drury | Promoted to active roster | | | Before |
| 8/29/2016 | Ari | Holland,D | Promoted to active roster | | | Before |
| 8/29/2016 | ChN | Minor | Released | | | Before |
| 8/29/2016 | LAN | Zych | Promoted to active roster | | | Before |
| 8/29/2016 | Pit | Bautista,J | Promoted to active roster | | | Before |
| 8/29/2016 | Pit | Kazmir | Demoted to reserve roster | | | Before |
| 8/29/2016 | Pit | Kingham | Promoted to active roster | | | Before |
| 8/29/2016 | Pit | Niese | Demoted to reserve roster | | | Before |
| 8/29/2016 | Pit | Parker,Jarre | Demoted to reserve roster | | | Before |
| 8/29/2016 | Pit | Young,CB | Promoted to active roster | | | Before |
| 8/29/2016 | SF | Cespedes | Promoted to active roster | | | Before |
| 8/29/2016 | SF | Goeddel,T | Demoted to reserve roster | | | Before |
| 8/29/2016 | SF | Peavy | Demoted to reserve roster | | | Before |
| 8/29/2016 | SF | Suarez,A | Promoted to active roster | | | Before |
| 8/31/2016 | SD | Rodriguez,Al | Demoted to reserve roster | | | Before |
| 9/1/2016 | Col | Morneau | Traded away | Tor | | After |
| 9/1/2016 | LAN | Colabello | Traded away | Phi | | After |
| 9/1/2016 | Phi | Colabello | Acquired via trade | LAN | | After |
| 9/1/2016 | Phi | Duke | Acquired via trade | ChA | | After |
| 9/1/2016 | Pit | Grimm | Traded away | NYA | | After |
| 9/1/2016 | Pit | Jackson,A | Traded away | Hou | | After |
| 9/1/2016 | Pit | Jimenez,U | Acquired via trade | Hou | | After |
| 9/1/2016 | Pit | Phillips,Bra | Acquired via trade | NYA | | After |
| 9/2/2016 | ChN | Greene,C | Promoted to active roster | | | Before |
| 9/2/2016 | ChN | Rivera,T | Promoted to active roster | | | Before |
| 9/2/2016 | Cin | Blackburn | Promoted to active roster | | | Before |
| 9/2/2016 | Cin | Collmenter | Promoted to active roster | | | Before |
| 9/2/2016 | Cin | Cuthbert | Promoted to active roster | | | Before |
| 9/2/2016 | Cin | Hardy,B | Promoted to active roster | | | Before |
| 9/2/2016 | Cin | Klein | Promoted to active roster | | | Before |
| 9/2/2016 | Cin | Plawecki | Promoted to active roster | | | Before |
| 9/2/2016 | Pit | Gilmartin | Signed | | | Before |
| 9/2/2016 | Pit | Hill,A | Signed | | | Before |
| 9/2/2016 | Pit | Niese | Released | | | Before |
| 9/2/2016 | Pit | Rzepczynski | Released | | | Before |
| 9/2/2016 | SF | Chacin | Signed | | | Before |
| 9/2/2016 | SF | Wilson,C | Released | | | Before |
| 9/2/2016 | StL | Gonzalez,S | Promoted to active roster | | | Before |
| 9/2/2016 | StL | Gyorko | Promoted to active roster | | | Before |
| 9/2/2016 | StL | Mieses | Promoted to active roster | | | Before |
| 9/2/2016 | StL | Peterson,Du | Promoted to active roster | | | Before |
| 9/2/2016 | StL | Santiago,H | Promoted to active roster | | | Before |
| 9/2/2016 | StL | Upton,M | Promoted to active roster | | | Before |
| 9/2/2016 | StL | Young,CR | Promoted to active roster | | | Before |
| 9/4/2016 | Cin | Almora | Promoted to active roster | | | Before |
| 9/4/2016 | Cin | Bethancourt | Demoted to reserve roster | | | Before |
| 9/4/2016 | Cin | Leake | Demoted to reserve roster | | | Before |
| 9/5/2016 | ChN | Guerra,Ju | Promoted to active roster | | | Before |
| 9/5/2016 | ChN | Nieuwenhuis | Signed | | | Before |
| 9/5/2016 | ChN | Nieuwenhuis | Promoted to active roster | | | Before |
| 9/5/2016 | ChN | Norris,B | Released | | | Before |
| 9/5/2016 | Col | Blair,A | Demoted to reserve roster | | | Before |
| 9/5/2016 | LAN | Parker,B | Signed | | | Before |
| 9/5/2016 | Pit | Broxton,J | Promoted to active roster | | | Before |
| 9/5/2016 | Pit | Gilmartin | Promoted to active roster | | | Before |
| 9/5/2016 | Pit | Hill,A | Promoted to active roster | | | Before |
| 9/5/2016 | Pit | Reynolds,Mar | Promoted to active roster | | | Before |
| 9/5/2016 | SF | Brown,T | Promoted to active roster | | | Before |
| 9/5/2016 | SF | Cain,M | Promoted to active roster | | | Before |
| 9/5/2016 | SF | Chacin | Promoted to active roster | | | Before |
| 9/5/2016 | SF | Doolittle | Promoted to active roster | | | Before |
| 9/5/2016 | SF | Goeddel,T | Promoted to active roster | | | Before |
| 9/5/2016 | SF | Gregorio | Promoted to active roster | | | Before |
| 9/5/2016 | StL | Adams,Ma | Promoted to active roster | | | Before |
| 9/5/2016 | StL | Teixeira | Released | | | Before |
| 9/5/2016 | StL | Walker,N | Demoted to reserve roster | | | Before |
| 9/6/2016 | Ari | Vizcaino | Demoted to reserve roster | | | Before |
| 9/8/2016 | Ari | Mitchell,B | Signed | | | Before |
| 9/8/2016 | Was | McCurry | Demoted to reserve roster | | | Before |
| 9/8/2016 | Was | Murphy,J | Released | | | Before |
| 9/8/2016 | Was | Teixeira | Signed | | | Before |
| 9/8/2016 | Was | Teixeira | Promoted to active roster | | | Before |
| 9/11/2016 | SF | Arrieta | Demoted to reserve roster | | | Before |
| 9/11/2016 | SF | Kluber | Demoted to reserve roster | | | Before |
| 9/12/2016 | ChN | Kang,J | Promoted to active roster | | | Before |
| 9/12/2016 | ChN | Keuchel | Demoted to reserve roster | | | Before |
| 9/12/2016 | LAN | Chafin | Promoted to active roster | | | Before |
| 9/12/2016 | LAN | Herrmann,C | Promoted to active roster | | | Before |
| 9/12/2016 | SD | de Aza | Promoted to active roster | | | Before |
| 9/12/2016 | SD | Gindl | Demoted to reserve roster | | | Before |
| 9/12/2016 | SD | Lopez,Jo | Promoted to active roster | | | Before |
| 9/12/2016 | SD | Miley | Promoted to active roster | | | Before |
| 9/12/2016 | SF | Arrieta | Promoted to active roster | | | Before |
| 9/12/2016 | SF | Kluber | Promoted to active roster | | | Before |
| 9/12/2016 | SF | Peavy | Promoted to active roster | | | Before |
| 9/12/2016 | SF | Rondon,H | Promoted to active roster | | | Before |
| 9/12/2016 | Was | Peterson,J | Signed | | | Before |
| 9/16/2016 | Pit | Ethier | Promoted to active roster | | | Before |
| 9/19/2016 | Cin | Hamilton,B | Demoted to reserve roster | | | Before |
| 9/19/2016 | Col | Reed,Co | Demoted to reserve roster | | | Before |
| 9/19/2016 | LAN | Jensen | Signed | | | Before |
| 9/19/2016 | Mil | Nova | Promoted to active roster | | | Before |
| 9/19/2016 | Mil | Sanchez,T | Released | | | Before |
| 9/19/2016 | Mil | Shoemaker,M | Demoted to reserve roster | | | Before |
| 9/19/2016 | Mil | Wendle | Signed | | | Before |
| 9/19/2016 | NYN | Henderson | Released | | | Before |
| 9/19/2016 | Pit | Diaz,El | Demoted to reserve roster | | | Before |
| 9/23/2016 | Cin | Leake | Promoted to active roster | | | Before |
| 9/26/2016 | ChN | Nieuwenhuis | Demoted to reserve roster | | | Before |
| 9/26/2016 | Cin | Michalczewski | Promoted to active roster | | | Before |
| 9/26/2016 | Cin | Souza | Demoted to reserve roster | | | Before |
| 9/26/2016 | LAN | Godley | Released | | | Before |
| 9/26/2016 | LAN | Gustave | Signed | | | Before |
| 9/26/2016 | LAN | Parker,B | Released | | | Before |
| 9/26/2016 | Mil | Walters,J | Released | | | Before |
| 9/26/2016 | StL | Diaz,Al | Promoted to active roster | | | Before |
| 9/30/2016 | Cin | Gonzalez,G | Demoted to reserve roster | | | Before |
| 9/30/2016 | Cin | McHugh | Demoted to reserve roster | | | Before |
| 9/30/2016 | Pit | Aybar | Released | | | Before |
| 9/30/2016 | Pit | Uribe | Released | | | Before |
| 9/30/2016 | StL | Cruz,T | Signed | | | Before |
| 9/30/2016 | StL | Cruz,T | Promoted to active roster | | | Before |
| 10/2/2016 | Pit | Gilmartin | Demoted to reserve roster | | | Before |
| 10/2/2016 | Pit | Holliday | Promoted to active roster | | | Before |
| 10/2/2016 | Pit | Jimenez,U | Demoted to reserve roster | | | Before |
| 10/2/2016 | Pit | Kingham | Demoted to reserve roster | | | Before |
| 10/2/2016 | Pit | Mercer | Demoted to reserve roster | | | Before |
| 10/2/2016 | Pit | Motte | Promoted to active roster | | | Before |
| 10/2/2016 | Pit | Reynolds,Mar | Demoted to reserve roster | | | Before |
| 10/2/2016 | Pit | Rondon,Jos | Demoted to reserve roster | | | Before |
