| Transactions |
| Date | Team | Player | Type | OthTm | EffDate | When |
| 4/5/2010 | BAL | Britton | Demoted to reserve roster | | | Before |
| 4/5/2010 | BAL | Duffy | Demoted to reserve roster | | | Before |
| 4/5/2010 | BAL | Hunter | Demoted to reserve roster | | | Before |
| 4/5/2010 | BAL | Hunter | Promoted to active roster | | | Before |
| 4/5/2010 | BAL | Lawrie | Demoted to reserve roster | | | Before |
| 4/5/2010 | BAL | Litsch | Demoted to reserve roster | | | Before |
| 4/5/2010 | BAL | Moustakas | Demoted to reserve roster | | | Before |
| 4/5/2010 | BAL | Murphy | Demoted to reserve roster | | | Before |
| 4/5/2010 | BAL | Zimmermann | Demoted to reserve roster | | | Before |
| 4/5/2010 | OAK | Meche | Demoted to reserve roster | | | Before |
| 4/5/2010 | TEX | Condrey | Demoted to reserve roster | | | Before |
| 4/5/2010 | TEX | Gabbard | Promoted to active roster | | | Before |
| 4/5/2010 | TEX | Gonzalez | Promoted to active roster | | | Before |
| 4/5/2010 | TEX | Wade | Demoted to reserve roster | | | Before |
| 4/5/2010 | TEX | Wood | Demoted to reserve roster | | | Before |
| 4/8/2010 | DET | Jakubauskas | Acquired via trade | PIT | | After |
| 4/9/2010 | ANA | Jones | Released | | | Before |
| 4/9/2010 | ANA | Sheffield | Released | | | Before |
| 4/9/2010 | BOS | Castro | Released | | | Before |
| 4/9/2010 | BOS | Johnson | Signed | | | Before |
| 4/9/2010 | BOS | Johnson | Promoted to active roster | | | Before |
| 4/9/2010 | CHA | Danks | Demoted to reserve roster | | | Before |
| 4/9/2010 | CHA | Danks | Promoted to active roster | | | Before |
| 4/9/2010 | CHA | Niemann | Demoted to reserve roster | | | Before |
| 4/9/2010 | CHA | Reimold | Promoted to active roster | | | Before |
| 4/9/2010 | DET | Jakubauskas | Promoted to active roster | | | Before |
| 4/10/2010 | OAK | Wellemeyer | Promoted to active roster | | | Before |
| 4/12/2010 | BOS | Beavan | Promoted to active roster | | | Before |
| 4/12/2010 | BOS | Bell | Promoted to active roster | | | Before |
| 4/12/2010 | BOS | Caridad | Released | | | Before |
| 4/12/2010 | BOS | Carter | Promoted to active roster | | | Before |
| 4/12/2010 | BOS | Hinske | Released | | | Before |
| 4/12/2010 | BOS | Johnson | Released | | | Before |
| 4/12/2010 | OAK | Mayberry | Released | | | Before |
| 4/16/2010 | ANA | Guillen | Signed | | | Before |
| 4/16/2010 | ANA | Guillen | Promoted to active roster | | | Before |
| 4/16/2010 | ANA | Rivera | Demoted to reserve roster | | | Before |
| 4/16/2010 | BOS | Mortensen | Released | | | Before |
| 4/16/2010 | CHA | Aviles | Demoted to reserve roster | | | Before |
| 4/16/2010 | CHA | Niemann | Promoted to active roster | | | Before |
| 4/19/2010 | ANA | Bourjos | Demoted to reserve roster | | | Before |
| 4/19/2010 | ANA | Bourjos | Promoted to active roster | | | Before |
| 4/19/2010 | ANA | Crede | Demoted to reserve roster | | | Before |
| 4/19/2010 | ANA | Iorg | Demoted to reserve roster | | | Before |
| 4/19/2010 | ANA | Iorg | Promoted to active roster | | | Before |
| 4/19/2010 | ANA | Iorg | Promoted to active roster | | | Before |
| 4/19/2010 | BOS | Beavan | Demoted to reserve roster | | | Before |
| 4/19/2010 | BOS | Bell | Demoted to reserve roster | | | Before |
| 4/19/2010 | BOS | Hanrahan | Promoted to active roster | | | Before |
| 4/19/2010 | BOS | Mills | Released | | | Before |
| 4/19/2010 | BOS | Wilson | Signed | | | Before |
| 4/19/2010 | BOS | Wilson | Promoted to active roster | | | Before |
| 4/19/2010 | DET | Ascanio | Released | | | Before |
| 4/19/2010 | DET | Gonzalez | Demoted to reserve roster | | | Before |
| 4/19/2010 | DET | Leroux | Signed | | | Before |
| 4/19/2010 | DET | Mijares | Demoted to reserve roster | | | Before |
| 4/19/2010 | DET | Reyes | Promoted to active roster | | | Before |
| 4/19/2010 | DET | Rogers | Promoted to active roster | | | Before |
| 4/19/2010 | OAK | Lewis | Promoted to active roster | | | Before |
| 4/19/2010 | OAK | Meche | Promoted to active roster | | | Before |
| 4/19/2010 | OAK | Stanton | Demoted to reserve roster | | | Before |
| 4/19/2010 | OAK | Wellemeyer | Demoted to reserve roster | | | Before |
| 4/19/2010 | SEA | Cameron | Demoted to reserve roster | | | Before |
| 4/19/2010 | SEA | Galarraga | Promoted to active roster | | | Before |
| 4/19/2010 | SEA | Mickolio | Promoted to active roster | | | Before |
| 4/19/2010 | SEA | Mock | Demoted to reserve roster | | | Before |
| 4/19/2010 | SEA | Swindle | Demoted to reserve roster | | | Before |
| 4/20/2010 | NYA | Rowand | Demoted to reserve roster | | | Before |
| 4/22/2010 | ANA | Arroyo | Acquired via trade | CIN | | After |
| 4/22/2010 | ANA | Jones | Traded away | CIN | | After |
| 4/22/2010 | DET | Fox | Acquired via trade | ARI | | After |
| 4/22/2010 | DET | Gaub | Traded away | ARI | | After |
| 4/23/2010 | ANA | Bourjos | Demoted to reserve roster | | | Before |
| 4/23/2010 | ANA | Bourjos | Promoted to active roster | | | Before |
| 4/23/2010 | ANA | Jacobs | Released | | | Before |
| 4/23/2010 | ANA | Saunders | Demoted to reserve roster | | | Before |
| 4/23/2010 | ANA | Varitek | Promoted to active roster | | | Before |
| 4/23/2010 | BOS | Perez | Signed | | | Before |
| 4/23/2010 | KC | Cain | Signed | | | Before |
| 4/23/2010 | KC | Hoffpauir | Released | | | Before |
| 4/23/2010 | OAK | Wise | Signed | | | Before |
| 4/23/2010 | TEX | Green | Demoted to reserve roster | | | Before |
| 4/23/2010 | TEX | Morse | Demoted to reserve roster | | | Before |
| 4/26/2010 | ANA | Bourjos | Promoted to active roster | | | Before |
| 4/26/2010 | ANA | Punto | Demoted to reserve roster | | | Before |
| 4/26/2010 | ANA | Ramirez | Demoted to reserve roster | | | Before |
| 4/26/2010 | ANA | Trumbo | Promoted to active roster | | | Before |
| 4/26/2010 | BOS | Bell | Demoted to reserve roster | | | Before |
| 4/26/2010 | BOS | Bell | Promoted to active roster | | | Before |
| 4/26/2010 | BOS | Cahill | Promoted to active roster | | | Before |
| 4/26/2010 | BOS | Cowgill | Signed | | | Before |
| 4/26/2010 | BOS | Cowgill | Promoted to active roster | | | Before |
| 4/26/2010 | BOS | Perdomo | Released | | | Before |
| 4/26/2010 | BOS | Perez | Released | | | Before |
| 4/26/2010 | BOS | Snyder | Promoted to active roster | | | Before |
| 4/26/2010 | BOS | Strieby | Demoted to reserve roster | | | Before |
| 4/26/2010 | BOS | Wilson | Released | | | Before |
| 4/26/2010 | MIN | Gaudin | Signed | | | Before |
| 4/26/2010 | MIN | Gaudin | Promoted to active roster | | | Before |
| 4/26/2010 | MIN | Happ | Demoted to reserve roster | | | Before |
| 4/26/2010 | MIN | Hicks | Released | | | Before |
| 4/26/2010 | OAK | Cameron | Demoted to reserve roster | | | Before |
| 4/26/2010 | OAK | Descalso | Demoted to reserve roster | | | Before |
| 4/26/2010 | OAK | Descalso | Promoted to active roster | | | Before |
| 4/26/2010 | OAK | Ellis | Demoted to reserve roster | | | Before |
| 4/26/2010 | OAK | Lugo | Promoted to active roster | | | Before |
| 4/26/2010 | OAK | Wise | Promoted to active roster | | | Before |
| 4/26/2010 | SEA | Berkman | Promoted to active roster | | | Before |
| 4/26/2010 | TB | Ellsbury | Demoted to reserve roster | | | Before |
| 4/26/2010 | TB | Encarnacion | Demoted to reserve roster | | | Before |
| 4/26/2010 | TOR | Branyan | Promoted to active roster | | | Before |
| 4/26/2010 | TOR | Guillen | Demoted to reserve roster | | | Before |
| 4/27/2010 | NYA | Igarashi | Demoted to reserve roster | | | Before |
| 4/29/2010 | KC | Lopez | Acquired via trade | CIN | | After |
| 4/30/2010 | BOS | Brown | Released | | | Before |
| 4/30/2010 | BOS | Brown | Signed | | | Before |
| 4/30/2010 | BOS | Nova | Signed | | | Before |
| 4/30/2010 | DET | Doolittle | Signed | | | Before |
| 4/30/2010 | DET | Martinez | Released | | | Before |
| 4/30/2010 | DET | Nunez | Signed | | | Before |
| 4/30/2010 | MIN | Gaudin | Released | | | Before |
| 4/30/2010 | MIN | Gaudin | Signed | | | Before |
| 4/30/2010 | MIN | Haeger | Released | | | Before |
| 4/30/2010 | MIN | Haeger | Signed | | | Before |
| 4/30/2010 | MIN | Kulbacki | Released | | | Before |
| 4/30/2010 | MIN | Robinson | Signed | | | Before |
| 4/30/2010 | OAK | Descalso | Promoted to active roster | | | Before |
| 4/30/2010 | OAK | Lugo | Demoted to reserve roster | | | Before |
| 5/2/2010 | ANA | Capps | Traded away | ARI | | After |
| 5/2/2010 | ANA | DeJesus | Traded away | ARI | | After |
| 5/2/2010 | ANA | Flowers | Acquired via trade | ARI | | After |
| 5/2/2010 | ANA | Kuroda | Traded away | ARI | | After |
| 5/2/2010 | ANA | McGee | Acquired via trade | ARI | | After |
| 5/2/2010 | ANA | Viciedo | Acquired via trade | ARI | | After |
| 5/3/2010 | BOS | Cowgill | Released | | | Before |
| 5/3/2010 | BOS | Drabek | Demoted to reserve roster | | | Before |
| 5/3/2010 | BOS | Gaub | Signed | | | Before |
| 5/3/2010 | BOS | Gaub | Promoted to active roster | | | Before |
| 5/3/2010 | BOS | Nova | Released | | | Before |
| 5/3/2010 | BOS | Suomi | Signed | | | Before |
| 5/3/2010 | BOS | Suomi | Promoted to active roster | | | Before |
| 5/3/2010 | DET | Jakubauskas | Released | | | Before |
| 5/3/2010 | KC | Anderson | Demoted to reserve roster | | | Before |
| 5/3/2010 | KC | Cobb | Promoted to active roster | | | Before |
| 5/3/2010 | KC | Hunter | Promoted to active roster | | | Before |
| 5/3/2010 | MIN | Gaudin | Released | | | Before |
| 5/3/2010 | MIN | Haeger | Signed | | | Before |
| 5/3/2010 | MIN | Haeger | Promoted to active roster | | | Before |
| 5/3/2010 | MIN | Kimbrel | Demoted to reserve roster | | | Before |
| 5/3/2010 | MIN | Wuertz | Promoted to active roster | | | Before |
| 5/3/2010 | TB | Gross | Signed | | | Before |
| 5/3/2010 | TB | Suzuki | Demoted to reserve roster | | | Before |
| 5/3/2010 | TOR | Granderson | Demoted to reserve roster | | | Before |
| 5/3/2010 | TOR | Lee | Promoted to active roster | | | Before |
| 5/3/2010 | TOR | Wakefield | Demoted to reserve roster | | | Before |
| 5/3/2010 | TOR | Zaun | Promoted to active roster | | | Before |
| 5/4/2010 | TB | Gross | Signed | | | Before |
| 5/4/2010 | TB | Gross | Signed | | | Before |
| 5/4/2010 | TB | Gross | Promoted to active roster | | | Before |
| 5/4/2010 | TB | Suzuki | Demoted to reserve roster | | | Before |
| 5/4/2010 | TB | Suzuki | Demoted to reserve roster | | | Before |
| 5/5/2010 | CLE | Dickerson | Demoted to reserve roster | | | Before |
| 5/5/2010 | CLE | Padilla | Demoted to reserve roster | | | Before |
| 5/7/2010 | BOS | Bell | Promoted to active roster | | | Before |
| 5/7/2010 | BOS | Suomi | Released | | | Before |
| 5/7/2010 | CHA | Aviles | Promoted to active roster | | | Before |
| 5/7/2010 | CHA | Nippert | Demoted to reserve roster | | | Before |
| 5/7/2010 | CHA | Richardson | Demoted to reserve roster | | | Before |
| 5/7/2010 | CHA | Storen | Promoted to active roster | | | Before |
| 5/7/2010 | DET | Rosales | Signed | | | Before |
| 5/9/2010 | NYA | Beckett | Traded away | PIT | | After |
| 5/9/2010 | NYA | Feliz | Traded away | PIT | | After |
| 5/9/2010 | NYA | Halladay | Acquired via trade | PIT | | After |
| 5/9/2010 | NYA | Papelbon | Traded away | PIT | | After |
| 5/9/2010 | NYA | Stetter | Acquired via trade | PIT | | After |
| 5/9/2010 | NYA | Weathers | Acquired via trade | PIT | | After |
| 5/10/2010 | ANA | Medders | Promoted to active roster | | | Before |
| 5/10/2010 | ANA | Niese | Promoted to active roster | | | Before |
| 5/10/2010 | ANA | Punto | Promoted to active roster | | | Before |
| 5/10/2010 | ANA | Rivera | Promoted to active roster | | | Before |
| 5/10/2010 | ANA | Trumbo | Demoted to reserve roster | | | Before |
| 5/10/2010 | ANA | Varitek | Demoted to reserve roster | | | Before |
| 5/10/2010 | ANA | Viciedo | Promoted to active roster | | | Before |
| 5/10/2010 | DET | Bradley | Demoted to reserve roster | | | Before |
| 5/10/2010 | DET | Leroux | Promoted to active roster | | | Before |
| 5/10/2010 | MIN | Haeger | Promoted to active roster | | | Before |
| 5/10/2010 | MIN | Kimbrel | Demoted to reserve roster | | | Before |
| 5/10/2010 | MIN | Wuertz | Promoted to active roster | | | Before |
| 5/10/2010 | NYA | Carmona | Promoted to active roster | | | Before |
| 5/10/2010 | NYA | Rowand | Promoted to active roster | | | Before |
| 5/10/2010 | OAK | Gallagher | Demoted to reserve roster | | | Before |
| 5/10/2010 | OAK | Keppinger | Demoted to reserve roster | | | Before |
| 5/10/2010 | OAK | Keppinger | Promoted to active roster | | | Before |
| 5/10/2010 | OAK | Lugo | Promoted to active roster | | | Before |
| 5/10/2010 | OAK | Ross | Promoted to active roster | | | Before |
| 5/10/2010 | OAK | Tallet | Demoted to reserve roster | | | Before |
| 5/10/2010 | SEA | Ankiel | Demoted to reserve roster | | | Before |
| 5/10/2010 | SEA | Brown | Promoted to active roster | | | Before |
| 5/10/2010 | TB | Arredondo | Demoted to reserve roster | | | Before |
| 5/10/2010 | TB | Matsuzaka | Promoted to active roster | | | Before |
| 5/10/2010 | TB | Renteria | Demoted to reserve roster | | | Before |
| 5/10/2010 | TOR | Diamond | Released | | | Before |
| 5/10/2010 | TOR | Duchscherer | Demoted to reserve roster | | | Before |
| 5/10/2010 | TOR | Escobar | Demoted to reserve roster | | | Before |
| 5/10/2010 | TOR | Hawkins | Demoted to reserve roster | | | Before |
| 5/10/2010 | TOR | Meyers | Promoted to active roster | | | Before |
| 5/10/2010 | TOR | Mulligan | Promoted to active roster | | | Before |
| 5/10/2010 | TOR | Uehara | Promoted to active roster | | | Before |
| 5/11/2010 | SEA | Ankiel | Demoted to reserve roster | | | Before |
| 5/11/2010 | SEA | Brown | Promoted to active roster | | | Before |
| 5/11/2010 | TB | Nix | Released | | | Before |
| 5/11/2010 | TB | Nix | Signed | | | Before |
| 5/11/2010 | TB | Nix | Signed | | | Before |
| 5/13/2010 | ANA | Konerko | Traded away | CIN | | After |
| 5/13/2010 | ANA | Lewis | Traded away | CIN | | After |
| 5/13/2010 | ANA | Masterson | Acquired via trade | CIN | | After |
| 5/13/2010 | BOS | Walters | Signed | | | Before |
| 5/13/2010 | OAK | Wellemeyer | Released | | | Before |
| 5/13/2010 | SEA | Bogusevic | Signed | | | Before |
| 5/13/2010 | SEA | Cameron | Released | | | Before |
| 5/13/2010 | SEA | Rogers | Released | | | Before |
| 5/13/2010 | SEA | Webb | Signed | | | Before |
| 5/13/2010 | TB | Valdez | Released | | | Before |
| 5/17/2010 | CHA | Grabow | Demoted to reserve roster | | | Before |
| 5/17/2010 | CHA | Richardson | Promoted to active roster | | | Before |
| 5/17/2010 | DET | Mijares | Promoted to active roster | | | Before |
| 5/17/2010 | MIN | Barajas | Promoted to active roster | | | Before |
| 5/17/2010 | MIN | Francis | Promoted to active roster | | | Before |
| 5/17/2010 | MIN | Haeger | Demoted to reserve roster | | | Before |
| 5/17/2010 | MIN | Lewis | Promoted to active roster | | | Before |
| 5/17/2010 | MIN | Ramos | Demoted to reserve roster | | | Before |
| 5/17/2010 | MIN | Slama | Demoted to reserve roster | | | Before |
| 5/17/2010 | SEA | Swindle | Promoted to active roster | | | Before |
| 5/17/2010 | TEX | Byrnes | Demoted to reserve roster | | | Before |
| 5/17/2010 | TEX | Wood | Promoted to active roster | | | Before |
| 5/17/2010 | TOR | Clemens | Signed | | | Before |
| 5/17/2010 | TOR | Escobar | Promoted to active roster | | | Before |
| 5/17/2010 | TOR | Meyers | Demoted to reserve roster | | | Before |
| 5/17/2010 | TOR | Olson | Signed | | | Before |
| 5/17/2010 | TOR | Shouse | Released | | | Before |
| 5/17/2010 | TOR | Torres | Released | | | Before |
| 5/18/2010 | CLE | Carp | Demoted to reserve roster | | | Before |
| 5/18/2010 | CLE | Swarzak | Promoted to active roster | | | Before |
| 5/18/2010 | CLE | Wilson | Demoted to reserve roster | | | Before |
| 5/20/2010 | ANA | Ramirez | Promoted to active roster | | | Before |
| 5/21/2010 | BOS | Bell | Demoted to reserve roster | | | Before |
| 5/21/2010 | BOS | Burgess | Promoted to active roster | | | Before |
| 5/21/2010 | BOS | Deduno | Demoted to reserve roster | | | Before |
| 5/21/2010 | BOS | Strieby | Released | | | Before |
| 5/21/2010 | BOS | Wilson | Signed | | | Before |
| 5/21/2010 | BOS | Wilson | Promoted to active roster | | | Before |
| 5/21/2010 | CLE | Carp | Released | | | Before |
| 5/21/2010 | CLE | Wilson | Signed | | | Before |
| 5/21/2010 | CLE | Wilson | Promoted to active roster | | | Before |
| 5/21/2010 | DET | Leroux | Released | | | Before |
| 5/21/2010 | KC | Lopez | Promoted to active roster | | | Before |
| 5/21/2010 | KC | Sandoval | Demoted to reserve roster | | | Before |
| 5/21/2010 | MIN | Haeger | Released | | | Before |
| 5/21/2010 | MIN | Perkins | Released | | | Before |
| 5/21/2010 | MIN | Plouffe | Signed | | | Before |
| 5/21/2010 | OAK | Lugo | Demoted to reserve roster | | | Before |
| 5/21/2010 | OAK | Villone | Signed | | | Before |
| 5/21/2010 | OAK | Villone | Promoted to active roster | | | Before |
| 5/21/2010 | TOR | Gaston | Released | | | Before |
| 5/21/2010 | TOR | Patterson | Signed | | | Before |
| 5/24/2010 | ANA | Iorg | Released | | | Before |
| 5/24/2010 | ANA | Laird | Released | | | Before |
| 5/24/2010 | ANA | Lofgren | Promoted to active roster | | | Before |
| 5/24/2010 | ANA | McGee | Promoted to active roster | | | Before |
| 5/24/2010 | ANA | Medders | Demoted to reserve roster | | | Before |
| 5/24/2010 | ANA | Niese | Demoted to reserve roster | | | Before |
| 5/24/2010 | ANA | Reyes | Demoted to reserve roster | | | Before |
| 5/24/2010 | ANA | Reyes | Promoted to active roster | | | Before |
| 5/24/2010 | ANA | Rodriguez | Promoted to active roster | | | Before |
| 5/24/2010 | ANA | Saunders | Promoted to active roster | | | Before |
| 5/24/2010 | ANA | Sosa | Promoted to active roster | | | Before |
| 5/24/2010 | ANA | Varitek | Promoted to active roster | | | Before |
| 5/24/2010 | ANA | Viciedo | Demoted to reserve roster | | | Before |
| 5/24/2010 | ANA | Weathers | Demoted to reserve roster | | | Before |
| 5/24/2010 | BOS | Kilby | Signed | | | Before |
| 5/24/2010 | BOS | Kilby | Promoted to active roster | | | Before |
| 5/24/2010 | BOS | Wilson | Released | | | Before |
| 5/24/2010 | OAK | Descalso | Demoted to reserve roster | | | Before |
| 5/24/2010 | OAK | Ellis | Promoted to active roster | | | Before |
| 5/24/2010 | SEA | Cabrera | Demoted to reserve roster | | | Before |
| 5/24/2010 | TOR | Patterson | Promoted to active roster | | | Before |
| 5/24/2010 | TOR | Zaun | Demoted to reserve roster | | | Before |
| 5/25/2010 | DET | Bradley | Promoted to active roster | | | Before |
| 5/25/2010 | TEX | Young | Demoted to reserve roster | | | Before |
| 5/27/2010 | NYA | Montero | Promoted to active roster | | | Before |
| 5/27/2010 | NYA | Posada | Demoted to reserve roster | | | Before |
| 5/28/2010 | ANA | Dickey | Signed | | | Before |
| 5/28/2010 | ANA | Dickey | Promoted to active roster | | | Before |
| 5/28/2010 | ANA | McGee | Demoted to reserve roster | | | Before |
| 5/28/2010 | ANA | Soto | Signed | | | Before |
| 5/28/2010 | ANA | Weathers | Released | | | Before |
| 5/28/2010 | CHA | Dorn | Demoted to reserve roster | | | Before |
| 5/28/2010 | CHA | Schafer | Promoted to active roster | | | Before |
| 5/28/2010 | OAK | Martinez | Signed | | | Before |
| 5/31/2010 | DET | Wood | Signed | | | Before |
| 5/31/2010 | DET | Wood | Demoted to reserve roster | | | Before |
| 5/31/2010 | OAK | Cameron | Promoted to active roster | | | Before |
| 5/31/2010 | OAK | Gallagher | Promoted to active roster | | | Before |
| 5/31/2010 | OAK | Meche | Demoted to reserve roster | | | Before |
| 5/31/2010 | OAK | Ross | Demoted to reserve roster | | | Before |
| 5/31/2010 | SEA | Brown | Demoted to reserve roster | | | Before |
| 5/31/2010 | SEA | Sherrill | Demoted to reserve roster | | | Before |
| 5/31/2010 | SEA | Webb | Promoted to active roster | | | Before |
| 5/31/2010 | TOR | Granderson | Promoted to active roster | | | Before |
| 5/31/2010 | TOR | Guillen | Promoted to active roster | | | Before |
| 5/31/2010 | TOR | Hulett | Demoted to reserve roster | | | Before |
| 5/31/2010 | TOR | Hulett | Promoted to active roster | | | Before |
| 5/31/2010 | TOR | Patterson | Demoted to reserve roster | | | Before |
| 5/31/2010 | TOR | Uehara | Demoted to reserve roster | | | Before |
| 5/31/2010 | TOR | Wakefield | Demoted to reserve roster | | | Before |
| 5/31/2010 | TOR | Wakefield | Promoted to active roster | | | Before |
| 6/1/2010 | BAL | Lohse | Demoted to reserve roster | | | Before |
| 6/1/2010 | DET | Gillies | Demoted to reserve roster | | | Before |
| 6/1/2010 | KC | Anderson | Promoted to active roster | | | Before |
| 6/1/2010 | KC | Bannister | Demoted to reserve roster | | | Before |
| 6/4/2010 | BOS | Strop | Signed | | | Before |
| 6/4/2010 | BOS | Walters | Released | | | Before |
| 6/4/2010 | MIN | Gonzalez | Signed | | | Before |
| 6/4/2010 | OAK | Casilla | Signed | | | Before |
| 6/4/2010 | OAK | Lugo | Released | | | Before |
| 6/7/2010 | ANA | Thompson | Released | | | Before |
| 6/7/2010 | BOS | Kilby | Demoted to reserve roster | | | Before |
| 6/7/2010 | BOS | Wilson | Signed | | | Before |
| 6/7/2010 | BOS | Wilson | Promoted to active roster | | | Before |
| 6/7/2010 | CHA | Coffey | Demoted to reserve roster | | | Before |
| 6/7/2010 | CHA | Nippert | Promoted to active roster | | | Before |
| 6/7/2010 | KC | Anderson | Demoted to reserve roster | | | Before |
| 6/7/2010 | KC | Bannister | Promoted to active roster | | | Before |
| 6/7/2010 | KC | Bartlett | Demoted to reserve roster | | | Before |
| 6/7/2010 | KC | Cain | Promoted to active roster | | | Before |
| 6/7/2010 | KC | Cobb | Released | | | Before |
| 6/7/2010 | KC | Kottaras | Demoted to reserve roster | | | Before |
| 6/7/2010 | KC | Morales | Promoted to active roster | | | Before |
| 6/7/2010 | KC | Sandoval | Promoted to active roster | | | Before |
| 6/7/2010 | OAK | Casilla | Promoted to active roster | | | Before |
| 6/7/2010 | OAK | Cobb | Signed | | | Before |
| 6/7/2010 | OAK | Holland | Demoted to reserve roster | | | Before |
| 6/7/2010 | OAK | Tallet | Promoted to active roster | | | Before |
| 6/7/2010 | OAK | Villone | Released | | | Before |
| 6/7/2010 | SEA | Mock | Released | | | Before |
| 6/9/2010 | NYA | Montero | Demoted to reserve roster | | | Before |
| 6/9/2010 | NYA | Posada | Promoted to active roster | | | Before |
| 6/11/2010 | ANA | Burrell | Promoted to active roster | | | Before |
| 6/11/2010 | ANA | Lofgren | Demoted to reserve roster | | | Before |
| 6/11/2010 | ANA | Niese | Promoted to active roster | | | Before |
| 6/11/2010 | ANA | Sosa | Demoted to reserve roster | | | Before |
| 6/11/2010 | BOS | Wilson | Released | | | Before |
| 6/11/2010 | DET | Cecil | Promoted to active roster | | | Before |
| 6/11/2010 | DET | Wood | Promoted to active roster | | | Before |
| 6/11/2010 | TOR | Emaus | Signed | | | Before |
| 6/11/2010 | TOR | Mulligan | Demoted to reserve roster | | | Before |
| 6/11/2010 | TOR | Patterson | Released | | | Before |
| 6/11/2010 | TOR | Springer | Released | | | Before |
| 6/11/2010 | TOR | Wakefield | Promoted to active roster | | | Before |
| 6/13/2010 | OAK | McClellan | Acquired via trade | PHI | | After |
| 6/13/2010 | OAK | Qualls | Traded away | PHI | | After |
| 6/14/2010 | KC | Sandoval | Released | | | Before |
| 6/14/2010 | OAK | Baldelli | Released | | | Before |
| 6/14/2010 | OAK | Calero | Signed | | | Before |
| 6/14/2010 | OAK | Wagner | Released | | | Before |
| 6/14/2010 | SEA | Brown | Promoted to active roster | | | Before |
| 6/14/2010 | SEA | Quezada | Demoted to reserve roster | | | Before |
| 6/14/2010 | SEA | Sherrill | Promoted to active roster | | | Before |
| 6/14/2010 | SEA | Thames | Demoted to reserve roster | | | Before |
| 6/14/2010 | TB | Encarnacion | Promoted to active roster | | | Before |
| 6/14/2010 | TB | Hudson | Demoted to reserve roster | | | Before |
| 6/14/2010 | TB | Matsuzaka | Demoted to reserve roster | | | Before |
| 6/15/2010 | TEX | McLouth | Demoted to reserve roster | | | Before |
| 6/15/2010 | TEX | Morse | Promoted to active roster | | | Before |
| 6/15/2010 | TEX | Parraz | Promoted to active roster | | | Before |
| 6/18/2010 | ANA | Bonifacio | Demoted to reserve roster | | | Before |
| 6/18/2010 | ANA | Greene | Signed | | | Before |
| 6/18/2010 | ANA | Greene | Promoted to active roster | | | Before |
| 6/18/2010 | ANA | Lofgren | Released | | | Before |
| 6/18/2010 | BOS | Johnson | Signed | | | Before |
| 6/18/2010 | BOS | Strop | Released | | | Before |
| 6/18/2010 | CHA | Coffey | Promoted to active roster | | | Before |
| 6/18/2010 | CHA | Dorn | Promoted to active roster | | | Before |
| 6/18/2010 | CHA | Exposito | Demoted to reserve roster | | | Before |
| 6/18/2010 | CHA | Exposito | Promoted to active roster | | | Before |
| 6/18/2010 | CHA | Schafer | Demoted to reserve roster | | | Before |
| 6/18/2010 | CHA | Tatis | Demoted to reserve roster | | | Before |
| 6/18/2010 | OAK | Calero | Promoted to active roster | | | Before |
| 6/18/2010 | OAK | Cordero | Demoted to reserve roster | | | Before |
| 6/18/2010 | SEA | Bogusevic | Released | | | Before |
| 6/18/2010 | TEX | Betemit | Promoted to active roster | | | Before |
| 6/18/2010 | TEX | Byrnes | Promoted to active roster | | | Before |
| 6/18/2010 | TEX | Harden | Demoted to reserve roster | | | Before |
| 6/18/2010 | TEX | McLouth | Promoted to active roster | | | Before |
| 6/18/2010 | TOR | Mulligan | Promoted to active roster | | | Before |
| 6/18/2010 | TOR | Saito | Demoted to reserve roster | | | Before |
| 6/20/2010 | KC | Barton | Traded away | SF | | After |
| 6/20/2010 | KC | Pence | Acquired via trade | SF | | After |
| 6/20/2010 | TEX | Harden | Demoted to reserve roster | | | Before |
| 6/21/2010 | ANA | Medders | Released | | | Before |
| 6/21/2010 | KC | Bartlett | Promoted to active roster | | | Before |
| 6/24/2010 | OAK | Blake | Traded away | PHI | | After |
| 6/24/2010 | OAK | Francisco | Traded away | PHI | | After |
| 6/24/2010 | OAK | Ross | Acquired via trade | PHI | | After |
| 6/24/2010 | OAK | Vargas | Acquired via trade | PHI | | After |
| 6/24/2010 | TEX | McLouth | Demoted to reserve roster | | | Before |
| 6/25/2010 | BOS | Kilby | Released | | | Before |
| 6/25/2010 | BOS | Seay | Signed | | | Before |
| 6/25/2010 | OAK | Hall | Released | | | Before |
| 6/25/2010 | OAK | Luna | Signed | | | Before |
| 6/25/2010 | OAK | Luna | Promoted to active roster | | | Before |
| 6/25/2010 | OAK | Vargas | Promoted to active roster | | | Before |
| 6/25/2010 | TEX | Byrnes | Released | | | Before |
| 6/25/2010 | TOR | Jeroloman | Signed | | | Before |
| 6/25/2010 | TOR | Jeroloman | Promoted to active roster | | | Before |
| 6/25/2010 | TOR | Ruiz | Demoted to reserve roster | | | Before |
| 6/25/2010 | TOR | Zaun | Released | | | Before |
| 6/28/2010 | BOS | Chirinos | Signed | | | Before |
| 6/28/2010 | BOS | Seay | Released | | | Before |
| 6/28/2010 | DET | Fox | Released | | | Before |
| 6/28/2010 | MIN | Hall | Signed | | | Before |
| 6/28/2010 | MIN | Lewis | Released | | | Before |
| 6/28/2010 | OAK | Mulvey | Demoted to reserve roster | | | Before |
| 6/28/2010 | OAK | Street | Promoted to active roster | | | Before |
| 6/28/2010 | TOR | Uehara | Promoted to active roster | | | Before |
| 6/28/2010 | TOR | Wakefield | Demoted to reserve roster | | | Before |
| 7/1/2010 | BOS | Chirinos | Released | | | Before |
| 7/1/2010 | BOS | Murray | Released | | | Before |
| 7/1/2010 | OAK | Cordero | Released | | | Before |
| 7/1/2010 | OAK | Giese | Signed | | | Before |
| 7/1/2010 | SEA | Johnson | Released | | | Before |
| 7/1/2010 | TOR | Storey | Released | | | Before |
| 7/5/2010 | ANA | Bonifacio | Promoted to active roster | | | Before |
| 7/5/2010 | ANA | Flowers | Promoted to active roster | | | Before |
| 7/5/2010 | ANA | Ramirez | Demoted to reserve roster | | | Before |
| 7/5/2010 | ANA | Varitek | Demoted to reserve roster | | | Before |
| 7/5/2010 | BOS | Espineli | Released | | | Before |
| 7/5/2010 | BOS | Espineli | Signed | | | Before |
| 7/5/2010 | BOS | Gaub | Released | | | Before |
| 7/5/2010 | BOS | Johnson | Released | | | Before |
| 7/5/2010 | BOS | Mortensen | Signed | | | Before |
| 7/5/2010 | SEA | Freese | Demoted to reserve roster | | | Before |
| 7/5/2010 | TOR | Mulligan | Demoted to reserve roster | | | Before |
| 7/5/2010 | TOR | Saito | Promoted to active roster | | | Before |
| 7/6/2010 | DET | Braden | Demoted to reserve roster | | | Before |
| 7/6/2010 | DET | Utley | Demoted to reserve roster | | | Before |
| 7/6/2010 | NYA | Igarashi | Promoted to active roster | | | Before |
| 7/6/2010 | OAK | Giese | Promoted to active roster | | | Before |
| 7/6/2010 | OAK | Marcum | Demoted to reserve roster | | | Before |
| 7/9/2010 | BOS | Deduno | Released | | | Before |
| 7/9/2010 | BOS | Mortensen | Released | | | Before |
| 7/9/2010 | TOR | Chen | Signed | | | Before |
| 7/9/2010 | TOR | Olson | Released | | | Before |
| 7/15/2010 | ANA | Blalock | Demoted to reserve roster | | | Before |
| 7/15/2010 | ANA | Crede | Released | | | Before |
| 7/15/2010 | ANA | Reyes | Released | | | Before |
| 7/15/2010 | ANA | Trumbo | Promoted to active roster | | | Before |
| 7/15/2010 | BOS | Nunez | Signed | | | Before |
| 7/15/2010 | CHA | Coffey | Promoted to active roster | | | Before |
| 7/15/2010 | CHA | Dorn | Promoted to active roster | | | Before |
| 7/15/2010 | CHA | Schafer | Demoted to reserve roster | | | Before |
| 7/15/2010 | CHA | Tatis | Demoted to reserve roster | | | Before |
| 7/15/2010 | DET | Chirinos | Signed | | | Before |
| 7/15/2010 | DET | Cunningham | Signed | | | Before |
| 7/15/2010 | DET | Nunez | Released | | | Before |
| 7/15/2010 | KC | Cain | Demoted to reserve roster | | | Before |
| 7/15/2010 | KC | Crisp | Promoted to active roster | | | Before |
| 7/15/2010 | MIN | Happ | Promoted to active roster | | | Before |
| 7/15/2010 | TEX | Zambrano | Demoted to reserve roster | | | Before |
| 7/15/2010 | TOR | Johnson | Signed | | | Before |
| 7/19/2010 | ANA | Ramirez | Promoted to active roster | | | Before |
| 7/19/2010 | ANA | Trumbo | Demoted to reserve roster | | | Before |
| 7/19/2010 | BOS | Nunez | Released | | | Before |
| 7/19/2010 | DET | Chirinos | Promoted to active roster | | | Before |
| 7/19/2010 | DET | Wieters | Demoted to reserve roster | | | Before |
| 7/19/2010 | TB | Hudson | Demoted to reserve roster | | | Before |
| 7/19/2010 | TB | Hudson | Promoted to active roster | | | Before |
| 7/19/2010 | TB | Matsuzaka | Promoted to active roster | | | Before |
| 7/19/2010 | TB | Renteria | Promoted to active roster | | | Before |
| 7/19/2010 | TB | Suzuki | Promoted to active roster | | | Before |
| 7/19/2010 | TB | Talbot | Demoted to reserve roster | | | Before |
| 7/20/2010 | TEX | Wood | Demoted to reserve roster | | | Before |
| 7/20/2010 | TOR | Jeroloman | Demoted to reserve roster | | | Before |
| 7/20/2010 | TOR | Ruiz | Promoted to active roster | | | Before |
| 7/22/2010 | DET | Beckett | Acquired via trade | SF | | After |
| 7/22/2010 | DET | Lidge | Acquired via trade | SF | | After |
| 7/22/2010 | DET | Markakis | Traded away | SF | | After |
| 7/22/2010 | DET | Roberts | Acquired via trade | SF | | After |
| 7/23/2010 | CHA | Jimenez | Released | | | Before |
| 7/23/2010 | DET | Janish | Signed | | | Before |
| 7/23/2010 | DET | Nunez | Signed | | | Before |
| 7/23/2010 | DET | Wood | Released | | | Before |
| 7/23/2010 | TOR | Capuano | Signed | | | Before |
| 7/25/2010 | SEA | Morneau | Acquired via trade | ATL | | After |
| 7/25/2010 | SEA | Schlereth | Traded away | ATL | | After |
| 7/25/2010 | SEA | Sizemore | Traded away | ATL | | After |
| 7/26/2010 | ANA | Ramirez | Demoted to reserve roster | | | Before |
| 7/26/2010 | ANA | Soto | Promoted to active roster | | | Before |
| 7/26/2010 | BOS | Russell | Released | | | Before |
| 7/26/2010 | DET | Beckett | Promoted to active roster | | | Before |
| 7/26/2010 | DET | Braden | Promoted to active roster | | | Before |
| 7/26/2010 | DET | Cecil | Demoted to reserve roster | | | Before |
| 7/26/2010 | DET | Gonzalez | Promoted to active roster | | | Before |
| 7/26/2010 | DET | Roberts | Promoted to active roster | | | Before |
| 7/26/2010 | KC | Bogusevic | Signed | | | Before |
| 7/26/2010 | KC | Harrison | Promoted to active roster | | | Before |
| 7/26/2010 | KC | LaRoche | Demoted to reserve roster | | | Before |
| 7/26/2010 | KC | Lowrie | Promoted to active roster | | | Before |
| 7/26/2010 | KC | Robertson | Demoted to reserve roster | | | Before |
| 7/26/2010 | MIN | Inge | Demoted to reserve roster | | | Before |
| 7/26/2010 | MIN | Ramos | Promoted to active roster | | | Before |
| 7/26/2010 | OAK | Martinez | Released | | | Before |
| 7/26/2010 | SEA | Ankiel | Promoted to active roster | | | Before |
| 7/26/2010 | SEA | Cabrera | Promoted to active roster | | | Before |
| 7/26/2010 | SEA | Quezada | Promoted to active roster | | | Before |
| 7/26/2010 | SEA | Sheets | Demoted to reserve roster | | | Before |
| 7/26/2010 | SEA | Thames | Promoted to active roster | | | Before |
| 7/26/2010 | TOR | Guillen | Demoted to reserve roster | | | Before |
| 7/26/2010 | TOR | Johnson | Promoted to active roster | | | Before |
| 7/27/2010 | OAK | Doumit | Demoted to reserve roster | | | Before |
| 7/29/2010 | ANA | Arroyo | Traded away | BOS | | After |
| 7/29/2010 | BOS | Arroyo | Acquired via trade | ANA | | After |
| 7/29/2010 | NYA | Hamilton | Acquired via trade | CIN | | After |
| 7/29/2010 | NYA | Jennings | Traded away | CIN | | After |
| 7/29/2010 | NYA | McAllister | Acquired via trade | CIN | | After |
| 7/29/2010 | NYA | Vazquez | Traded away | CIN | | After |
| 7/30/2010 | ANA | Trumbo | Promoted to active roster | | | Before |
| 7/30/2010 | BOS | Espineli | Released | | | Before |
| 7/30/2010 | KC | Denorfia | Signed | | | Before |
| 7/30/2010 | KC | Espineli | Signed | | | Before |
| 7/30/2010 | KC | Patton | Released | | | Before |
| 7/30/2010 | KC | Robertson | Released | | | Before |
| 7/30/2010 | NYA | McAllister | Promoted to active roster | | | Before |
| 7/31/2010 | TEX | Velez | Demoted to reserve roster | | | Before |
| 8/1/2010 | DET | Drew | Acquired via trade | OAK | | After |
| 8/1/2010 | DET | Mazzaro | Traded away | OAK | | After |
| 8/1/2010 | DET | Roberts | Traded away | CIN | | After |
| 8/1/2010 | DET | Wood | Acquired via trade | CIN | | After |
| 8/1/2010 | OAK | Drew | Traded away | DET | | After |
| 8/1/2010 | OAK | Mazzaro | Acquired via trade | DET | | After |
| 8/2/2010 | ANA | Punto | Demoted to reserve roster | | | Before |
| 8/2/2010 | ANA | Sosa | Promoted to active roster | | | Before |
| 8/2/2010 | BOS | Cowgill | Signed | | | Before |
| 8/2/2010 | DET | Bradley | Demoted to reserve roster | | | Before |
| 8/2/2010 | DET | Wieters | Promoted to active roster | | | Before |
| 8/2/2010 | DET | Wood | Promoted to active roster | | | Before |
| 8/2/2010 | KC | Anderson | Promoted to active roster | | | Before |
| 8/2/2010 | KC | Espineli | Promoted to active roster | | | Before |
| 8/2/2010 | KC | Harrison | Demoted to reserve roster | | | Before |
| 8/2/2010 | KC | Manship | Demoted to reserve roster | | | Before |
| 8/2/2010 | MIN | Gonzalez | Released | | | Before |
| 8/2/2010 | MIN | Pineiro | Demoted to reserve roster | | | Before |
| 8/2/2010 | MIN | Sampson | Signed | | | Before |
| 8/2/2010 | MIN | Sampson | Promoted to active roster | | | Before |
| 8/2/2010 | OAK | Bay | Demoted to reserve roster | | | Before |
| 8/2/2010 | OAK | Descalso | Demoted to reserve roster | | | Before |
| 8/2/2010 | OAK | Descalso | Promoted to active roster | | | Before |
| 8/2/2010 | OAK | Gonzalez | Signed | | | Before |
| 8/2/2010 | OAK | Marcum | Promoted to active roster | | | Before |
| 8/2/2010 | OAK | Mazzaro | Demoted to reserve roster | | | Before |
| 8/2/2010 | OAK | Mulvey | Released | | | Before |
| 8/2/2010 | OAK | Stanton | Promoted to active roster | | | Before |
| 8/2/2010 | OAK | Wagner | Signed | | | Before |
| 8/2/2010 | OAK | Wagner | Promoted to active roster | | | Before |
| 8/2/2010 | SEA | Rowland-Smith | Demoted to reserve roster | | | Before |
| 8/2/2010 | TOR | Fisher | Demoted to reserve roster | | | Before |
| 8/2/2010 | TOR | Hawkins | Promoted to active roster | | | Before |
| 8/3/2010 | TEX | Harden | Promoted to active roster | | | Before |
| 8/3/2010 | TEX | Moyer | Demoted to reserve roster | | | Before |
| 8/3/2010 | TEX | Wood | Promoted to active roster | | | Before |
| 8/3/2010 | TEX | Zambrano | Promoted to active roster | | | Before |
| 8/5/2010 | ANA | McGee | Promoted to active roster | | | Before |
| 8/5/2010 | ANA | Wolf | Traded away | MIN | | After |
| 8/5/2010 | BOS | Snyder | Released | | | Before |
| 8/5/2010 | CLE | Crowe | Promoted to active roster | | | Before |
| 8/5/2010 | CLE | Dickerson | Promoted to active roster | | | Before |
| 8/5/2010 | CLE | Ellis | Demoted to reserve roster | | | Before |
| 8/5/2010 | CLE | Florimon | Promoted to active roster | | | Before |
| 8/5/2010 | CLE | Jaso | Promoted to active roster | | | Before |
| 8/5/2010 | CLE | Padilla | Promoted to active roster | | | Before |
| 8/5/2010 | CLE | Ramirez | Demoted to reserve roster | | | Before |
| 8/5/2010 | CLE | Sowers | Demoted to reserve roster | | | Before |
| 8/5/2010 | CLE | Sowers | Promoted to active roster | | | Before |
| 8/5/2010 | CLE | Swarzak | Demoted to reserve roster | | | Before |
| 8/5/2010 | CLE | Vitters | Demoted to reserve roster | | | Before |
| 8/5/2010 | CLE | Wilson | Promoted to active roster | | | Before |
| 8/5/2010 | KC | Anderson | Traded away | ARI | | After |
| 8/5/2010 | KC | Fister | Acquired via trade | ARI | | After |
| 8/5/2010 | KC | Frasor | Traded away | ARI | | After |
| 8/5/2010 | KC | Perdomo | Acquired via trade | ARI | | After |
| 8/5/2010 | KC | Wigginton | Traded away | WAS | | After |
| 8/5/2010 | MIN | Barajas | Demoted to reserve roster | | | Before |
| 8/5/2010 | MIN | DeLome | Released | | | Before |
| 8/5/2010 | MIN | Donaldson | Promoted to active roster | | | Before |
| 8/5/2010 | MIN | Lo | Released | | | Before |
| 8/5/2010 | MIN | Sampson | Demoted to reserve roster | | | Before |
| 8/5/2010 | MIN | Schlichting | Signed | | | Before |
| 8/5/2010 | MIN | Tejeda | Signed | | | Before |
| 8/5/2010 | MIN | Wolf | Acquired via trade | ANA | | After |
| 8/5/2010 | SEA | Riske | Signed | | | Before |
| 8/5/2010 | TOR | Diamond | Signed | | | Before |
| 8/9/2010 | CHA | Tatis | Released | | | Before |
| 8/9/2010 | CHA | Youkilis | Demoted to reserve roster | | | Before |
| 8/9/2010 | MIN | Donaldson | Demoted to reserve roster | | | Before |
| 8/9/2010 | MIN | Inge | Promoted to active roster | | | Before |
| 8/9/2010 | NYA | Howard | Demoted to reserve roster | | | Before |
| 8/9/2010 | OAK | Doumit | Promoted to active roster | | | Before |
| 8/9/2010 | OAK | Wagner | Demoted to reserve roster | | | Before |
| 8/9/2010 | SEA | Joyce | Promoted to active roster | | | Before |
| 8/9/2010 | SEA | Morgan | Demoted to reserve roster | | | Before |
| 8/9/2010 | SEA | Riske | Promoted to active roster | | | Before |
| 8/9/2010 | SEA | Worrell | Demoted to reserve roster | | | Before |
| 8/9/2010 | TOR | Emaus | Promoted to active roster | | | Before |
| 8/9/2010 | TOR | Pena | Demoted to reserve roster | | | Before |
| 8/9/2010 | TOR | Sonnanstine | Demoted to reserve roster | | | Before |
| 8/9/2010 | TOR | Wakefield | Promoted to active roster | | | Before |
| 8/10/2010 | BAL | Gomez | Demoted to reserve roster | | | Before |
| 8/10/2010 | CHA | Nippert | Demoted to reserve roster | | | Before |
| 8/10/2010 | OAK | Cameron | Demoted to reserve roster | | | Before |
| 8/10/2010 | OAK | Descalso | Promoted to active roster | | | Before |
| 8/10/2010 | OAK | Giese | Demoted to reserve roster | | | Before |
| 8/12/2010 | CHA | Arrieta | Traded away | SF | | After |
| 8/12/2010 | CHA | Barton | Acquired via trade | SF | | After |
| 8/12/2010 | CHA | Headley | Acquired via trade | SF | | After |
| 8/12/2010 | CHA | Peavy | Acquired via trade | SF | | After |
| 8/12/2010 | CHA | Rios | Acquired via trade | SF | | After |
| 8/12/2010 | CHA | Smoak | Traded away | SF | | After |
| 8/12/2010 | CHA | Zimmerman | Traded away | SF | | After |
| 8/12/2010 | CLE | Jaso | Traded away | KC | | After |
| 8/12/2010 | CLE | Wallace | Acquired via trade | KC | | After |
| 8/12/2010 | CLE | Wells | Traded away | KC | | After |
| 8/12/2010 | KC | Jaso | Acquired via trade | CLE | | After |
| 8/12/2010 | KC | Wallace | Traded away | CLE | | After |
| 8/12/2010 | KC | Wells | Acquired via trade | CLE | | After |
| 8/13/2010 | CLE | Arencibia | Demoted to reserve roster | | | Before |
| 8/13/2010 | CLE | Arencibia | Promoted to active roster | | | Before |
| 8/13/2010 | CLE | Arencibia | Promoted to active roster | | | Before |
| 8/13/2010 | CLE | Darnell | Promoted to active roster | | | Before |
| 8/13/2010 | CLE | Dickerson | Demoted to reserve roster | | | Before |
| 8/13/2010 | CLE | Ellis | Promoted to active roster | | | Before |
| 8/13/2010 | CLE | Powell | Demoted to reserve roster | | | Before |
| 8/13/2010 | CLE | Powell | Promoted to active roster | | | Before |
| 8/13/2010 | CLE | Wallace | Promoted to active roster | | | Before |
| 8/13/2010 | CLE | Wilson | Demoted to reserve roster | | | Before |
| 8/13/2010 | KC | Fister | Promoted to active roster | | | Before |
| 8/13/2010 | KC | Jaso | Demoted to reserve roster | | | Before |
| 8/13/2010 | KC | Perdomo | Promoted to active roster | | | Before |
| 8/13/2010 | OAK | Giese | Released | | | Before |
| 8/13/2010 | OAK | Wagner | Released | | | Before |
| 8/15/2010 | CLE | Gomez | Acquired via trade | WAS | | After |
| 8/15/2010 | CLE | Padilla | Traded away | WAS | | After |
| 8/15/2010 | CLE | Venable | Acquired via trade | WAS | | After |
| 8/16/2010 | CHA | Niemann | Demoted to reserve roster | | | Before |
| 8/16/2010 | CLE | Crowe | Demoted to reserve roster | | | Before |
| 8/16/2010 | CLE | Purcey | Promoted to active roster | | | Before |
| 8/16/2010 | CLE | Venable | Promoted to active roster | | | Before |
| 8/16/2010 | DET | Mijares | Demoted to reserve roster | | | Before |
| 8/16/2010 | DET | Nunez | Released | | | Before |
| 8/16/2010 | DET | Strop | Signed | | | Before |
| 8/16/2010 | KC | Guzman | Demoted to reserve roster | | | Before |
| 8/16/2010 | KC | LaRoche | Promoted to active roster | | | Before |
| 8/16/2010 | MIN | Duncan | Demoted to reserve roster | | | Before |
| 8/16/2010 | MIN | Francis | Demoted to reserve roster | | | Before |
| 8/16/2010 | MIN | Gillaspie | Released | | | Before |
| 8/16/2010 | MIN | Gillaspie | Signed | | | Before |
| 8/16/2010 | MIN | Sampson | Released | | | Before |
| 8/16/2010 | MIN | Sampson | Signed | | | Before |
| 8/16/2010 | MIN | Sampson | Promoted to active roster | | | Before |
| 8/16/2010 | MIN | Teahen | Promoted to active roster | | | Before |
| 8/16/2010 | OAK | Calero | Released | | | Before |
| 8/16/2010 | OAK | Holland | Promoted to active roster | | | Before |
| 8/16/2010 | OAK | Keppinger | Promoted to active roster | | | Before |
| 8/16/2010 | SEA | Delgado | Promoted to active roster | | | Before |
| 8/16/2010 | TB | Ellsbury | Promoted to active roster | | | Before |
| 8/16/2010 | TB | Perez | Demoted to reserve roster | | | Before |
| 8/16/2010 | TB | Renteria | Demoted to reserve roster | | | Before |
| 8/16/2010 | TEX | Green | Promoted to active roster | | | Before |
| 8/16/2010 | TEX | Harden | Demoted to reserve roster | | | Before |
| 8/16/2010 | TEX | McLouth | Promoted to active roster | | | Before |
| 8/16/2010 | TEX | Wade | Promoted to active roster | | | Before |
| 8/16/2010 | TEX | Young | Promoted to active roster | | | Before |
| 8/18/2010 | TEX | Cabrera | Demoted to reserve roster | | | Before |
| 8/19/2010 | SEA | Johnson | Traded away | NYN | | After |
| 8/19/2010 | SEA | Lowe | Acquired via trade | NYN | | After |
| 8/19/2010 | SEA | Scarpetta | Traded away | NYN | | After |
| 8/19/2010 | SEA | Shields | Acquired via trade | NYN | | After |
| 8/19/2010 | SEA | Villanueva | Acquired via trade | NYN | | After |
| 8/20/2010 | CHA | Nippert | Released | | | Before |
| 8/20/2010 | CLE | Cousins | Signed | | | Before |
| 8/20/2010 | CLE | French | Signed | | | Before |
| 8/20/2010 | CLE | Hurley | Released | | | Before |
| 8/20/2010 | CLE | Larish | Signed | | | Before |
| 8/20/2010 | CLE | Liz | Released | | | Before |
| 8/20/2010 | CLE | Martis | Released | | | Before |
| 8/20/2010 | CLE | Powell | Released | | | Before |
| 8/20/2010 | CLE | Ramirez | Released | | | Before |
| 8/20/2010 | CLE | Richmond | Signed | | | Before |
| 8/20/2010 | CLE | Sogard | Signed | | | Before |
| 8/20/2010 | CLE | Sowers | Released | | | Before |
| 8/20/2010 | CLE | Swarzak | Released | | | Before |
| 8/20/2010 | CLE | Towles | Signed | | | Before |
| 8/20/2010 | CLE | Wilson | Released | | | Before |
| 8/20/2010 | OAK | Bozied | Signed | | | Before |
| 8/20/2010 | OAK | Bozied | Promoted to active roster | | | Before |
| 8/20/2010 | OAK | Keppinger | Demoted to reserve roster | | | Before |
| 8/20/2010 | SEA | Mickolio | Demoted to reserve roster | | | Before |
| 8/20/2010 | SEA | Riske | Released | | | Before |
| 8/20/2010 | SEA | Worrell | Released | | | Before |
| 8/20/2010 | TOR | Emaus | Demoted to reserve roster | | | Before |
| 8/20/2010 | TOR | Fisher | Promoted to active roster | | | Before |
| 8/20/2010 | TOR | Guillen | Promoted to active roster | | | Before |
| 8/20/2010 | TOR | Hawkins | Released | | | Before |
| 8/22/2010 | BOS | Stevens | Traded away | NYA | | After |
| 8/22/2010 | KC | Capps | Acquired via trade | ARI | | After |
| 8/22/2010 | KC | Damon | Traded away | ARI | | After |
| 8/22/2010 | NYA | Stevens | Acquired via trade | BOS | | After |
| 8/23/2010 | ANA | Abad | Signed | | | Before |
| 8/23/2010 | ANA | Abad | Promoted to active roster | | | Before |
| 8/23/2010 | ANA | Greene | Demoted to reserve roster | | | Before |
| 8/23/2010 | ANA | Ramirez | Promoted to active roster | | | Before |
| 8/23/2010 | ANA | Rodriguez | Demoted to reserve roster | | | Before |
| 8/23/2010 | KC | Bogusevic | Promoted to active roster | | | Before |
| 8/23/2010 | KC | Carrasco | Demoted to reserve roster | | | Before |
| 8/23/2010 | KC | Castro | Demoted to reserve roster | | | Before |
| 8/23/2010 | KC | Denorfia | Demoted to reserve roster | | | Before |
| 8/23/2010 | KC | Denorfia | Promoted to active roster | | | Before |
| 8/23/2010 | KC | Jaso | Promoted to active roster | | | Before |
| 8/23/2010 | MIN | Barajas | Promoted to active roster | | | Before |
| 8/23/2010 | MIN | Kimbrel | Promoted to active roster | | | Before |
| 8/23/2010 | MIN | Mahay | Demoted to reserve roster | | | Before |
| 8/23/2010 | MIN | Ramos | Demoted to reserve roster | | | Before |
| 8/23/2010 | NYA | Howard | Promoted to active roster | | | Before |
| 8/23/2010 | NYA | Rodriguez | Demoted to reserve roster | | | Before |
| 8/23/2010 | NYA | Septimo | Released | | | Before |
| 8/23/2010 | SEA | Berkman | Demoted to reserve roster | | | Before |
| 8/23/2010 | SEA | Delgado | Demoted to reserve roster | | | Before |
| 8/23/2010 | SEA | Morgan | Promoted to active roster | | | Before |
| 8/23/2010 | TOR | Guillen | Demoted to reserve roster | | | Before |
| 8/23/2010 | TOR | Pena | Promoted to active roster | | | Before |
| 8/23/2010 | TOR | Sonnanstine | Promoted to active roster | | | Before |
| 8/23/2010 | TOR | Wakefield | Demoted to reserve roster | | | Before |
| 8/26/2010 | ANA | Cobb | Acquired via trade | OAK | | After |
| 8/26/2010 | ANA | Ramirez | Traded away | OAK | | After |
| 8/26/2010 | OAK | Cobb | Traded away | ANA | | After |
| 8/26/2010 | OAK | Ramirez | Acquired via trade | ANA | | After |
| 8/27/2010 | ANA | Blalock | Released | | | Before |
| 8/27/2010 | ANA | Cobb | Promoted to active roster | | | Before |
| 8/27/2010 | CLE | Ambriz | Promoted to active roster | | | Before |
| 8/27/2010 | CLE | Bastardo | Demoted to reserve roster | | | Before |
| 8/27/2010 | CLE | Crowe | Promoted to active roster | | | Before |
| 8/27/2010 | CLE | French | Promoted to active roster | | | Before |
| 8/27/2010 | CLE | Gomez | Promoted to active roster | | | Before |
| 8/27/2010 | CLE | Larish | Promoted to active roster | | | Before |
| 8/27/2010 | CLE | Purcey | Demoted to reserve roster | | | Before |
| 8/27/2010 | CLE | Ramirez | Promoted to active roster | | | Before |
| 8/27/2010 | CLE | Sogard | Promoted to active roster | | | Before |
| 8/27/2010 | OAK | Vargas | Demoted to reserve roster | | | Before |
| 8/30/2010 | ANA | Baez | Demoted to reserve roster | | | Before |
| 8/30/2010 | ANA | Greene | Promoted to active roster | | | Before |
| 8/30/2010 | BOS | Johnson | Signed | | | Before |
| 8/30/2010 | CLE | Wilson | Released | | | Before |
| 8/30/2010 | DET | Utley | Promoted to active roster | | | Before |
| 8/30/2010 | TOR | Mulligan | Promoted to active roster | | | Before |
| 8/30/2010 | TOR | Thornton | Demoted to reserve roster | | | Before |
| 9/2/2010 | CLE | Guerrero | Traded away | PIT | | After |
| 9/2/2010 | CLE | Nova | Acquired via trade | PIT | | After |
| 9/2/2010 | DET | Cunningham | Traded away | MIL | | After |
| 9/2/2010 | DET | Watson | Acquired via trade | MIL | | After |
| 9/2/2010 | KC | Bartlett | Traded away | CIN | | After |
| 9/2/2010 | KC | Castro | Traded away | HOU | | After |
| 9/2/2010 | KC | Hardy | Acquired via trade | CIN | | After |
| 9/2/2010 | KC | Hochevar | Acquired via trade | HOU | | After |
| 9/2/2010 | KC | Kalish | Acquired via trade | HOU | | After |
| 9/2/2010 | MIN | Jakubauskas | Acquired via trade | STL | | After |
| 9/2/2010 | MIN | Teahen | Traded away | CHN | | After |
| 9/2/2010 | MIN | Wells | Acquired via trade | CHN | | After |
| 9/2/2010 | OAK | Reyes | Demoted to reserve roster | | | Before |
| 9/2/2010 | OAK | Meche | Traded away | TOR | | After |
| 9/2/2010 | TB | Encarnacion | Demoted to reserve roster | | | Before |
| 9/2/2010 | TOR | Meche | Acquired via trade | OAK | | After |
| 9/3/2010 | BOS | Bell | Promoted to active roster | | | Before |
| 9/3/2010 | BOS | Cowgill | Promoted to active roster | | | Before |
| 9/3/2010 | BOS | Drabek | Promoted to active roster | | | Before |
| 9/3/2010 | BOS | Hill | Signed | | | Before |
| 9/3/2010 | BOS | Hill | Promoted to active roster | | | Before |
| 9/3/2010 | BOS | Izturis | Released | | | Before |
| 9/3/2010 | BOS | Johnson | Promoted to active roster | | | Before |
| 9/3/2010 | BOS | Miller | Promoted to active roster | | | Before |
| 9/3/2010 | CLE | Arencibia | Demoted to reserve roster | | | Before |
| 9/3/2010 | CLE | Cousins | Promoted to active roster | | | Before |
| 9/3/2010 | CLE | Martinez | Released | | | Before |
| 9/3/2010 | CLE | Towles | Promoted to active roster | | | Before |
| 9/3/2010 | KC | Hochevar | Demoted to reserve roster | | | Before |
| 9/3/2010 | KC | Kalish | Demoted to reserve roster | | | Before |
| 9/3/2010 | MIN | Donaldson | Promoted to active roster | | | Before |
| 9/3/2010 | MIN | Duncan | Promoted to active roster | | | Before |
| 9/3/2010 | MIN | Jakubauskas | Promoted to active roster | | | Before |
| 9/3/2010 | MIN | Ramos | Promoted to active roster | | | Before |
| 9/3/2010 | MIN | Slama | Promoted to active roster | | | Before |
| 9/3/2010 | OAK | Gonzalez | Promoted to active roster | | | Before |
| 9/3/2010 | OAK | Mazzaro | Promoted to active roster | | | Before |
| 9/3/2010 | OAK | Neal | Promoted to active roster | | | Before |
| 9/3/2010 | OAK | Vargas | Promoted to active roster | | | Before |
| 9/3/2010 | TOR | Capuano | Promoted to active roster | | | Before |
| 9/3/2010 | TOR | Chen | Promoted to active roster | | | Before |
| 9/3/2010 | TOR | Choate | Signed | | | Before |
| 9/3/2010 | TOR | Choate | Promoted to active roster | | | Before |
| 9/3/2010 | TOR | Clemens | Promoted to active roster | | | Before |
| 9/3/2010 | TOR | Diamond | Promoted to active roster | | | Before |
| 9/3/2010 | TOR | Emaus | Promoted to active roster | | | Before |
| 9/3/2010 | TOR | Smoltz | Released | | | Before |
| 9/3/2010 | TOR | Wakefield | Promoted to active roster | | | Before |
| 9/6/2010 | CLE | Arencibia | Promoted to active roster | | | Before |
| 9/6/2010 | CLE | Florimon | Demoted to reserve roster | | | Before |
| 9/6/2010 | CLE | Gomez | Demoted to reserve roster | | | Before |
| 9/6/2010 | CLE | Purcey | Promoted to active roster | | | Before |
| 9/6/2010 | CLE | Wright | Released | | | Before |
| 9/6/2010 | KC | Carrasco | Promoted to active roster | | | Before |
| 9/6/2010 | KC | Denorfia | Promoted to active roster | | | Before |
| 9/6/2010 | KC | Guzman | Promoted to active roster | | | Before |
| 9/6/2010 | KC | Harrison | Promoted to active roster | | | Before |
| 9/6/2010 | KC | Hochevar | Promoted to active roster | | | Before |
| 9/6/2010 | KC | Kalish | Promoted to active roster | | | Before |
| 9/6/2010 | KC | Kottaras | Promoted to active roster | | | Before |
| 9/6/2010 | MIN | Gillaspie | Signed | | | Before |
| 9/6/2010 | MIN | Gillaspie | Demoted to reserve roster | | | Before |
| 9/6/2010 | MIN | Mahay | Released | | | Before |
| 9/6/2010 | MIN | Sampson | Released | | | Before |
| 9/6/2010 | OAK | Jimenez | Signed | | | Before |
| 9/6/2010 | SEA | Berkman | Promoted to active roster | | | Before |
| 9/6/2010 | SEA | Brown | Demoted to reserve roster | | | Before |
| 9/6/2010 | SEA | Rowland-Smith | Promoted to active roster | | | Before |
| 9/6/2010 | TOR | Meche | Promoted to active roster | | | Before |
| 9/10/2010 | ANA | Durango | Signed | | | Before |
| 9/10/2010 | ANA | Durango | Promoted to active roster | | | Before |
| 9/10/2010 | BOS | Castillo | Signed | | | Before |
| 9/10/2010 | BOS | Johnson | Released | | | Before |
| 9/10/2010 | BOS | Rogers | Signed | | | Before |
| 9/10/2010 | CLE | Bond | Signed | | | Before |
| 9/10/2010 | CLE | Bond | Promoted to active roster | | | Before |
| 9/10/2010 | CLE | Green | Signed | | | Before |
| 9/10/2010 | CLE | Huffman | Released | | | Before |
| 9/10/2010 | CLE | Hurley | Signed | | | Before |
| 9/10/2010 | CLE | Reynolds | Signed | | | Before |
| 9/10/2010 | MIN | Francis | Promoted to active roster | | | Before |
| 9/10/2010 | MIN | Hall | Promoted to active roster | | | Before |
| 9/10/2010 | OAK | Jimenez | Promoted to active roster | | | Before |
| 9/13/2010 | ANA | Punto | Promoted to active roster | | | Before |
| 9/13/2010 | ANA | Varitek | Promoted to active roster | | | Before |
| 9/13/2010 | CHA | Niemann | Promoted to active roster | | | Before |
| 9/13/2010 | CLE | Florimon | Released | | | Before |
| 9/13/2010 | DET | Cecil | Promoted to active roster | | | Before |
| 9/13/2010 | DET | Janish | Promoted to active roster | | | Before |
| 9/13/2010 | DET | Mijares | Promoted to active roster | | | Before |
| 9/13/2010 | TOR | Thornton | Promoted to active roster | | | Before |
| 9/21/2010 | NYA | Rodriguez | Promoted to active roster | | | Before |
| 9/24/2010 | BOS | Iwamura | Released | | | Before |
| 9/24/2010 | BOS | Johnson | Signed | | | Before |
| 9/24/2010 | BOS | Miller | Released | | | Before |
| 9/26/2010 | TB | Ellsbury | Demoted to reserve roster | | | Before |
| 9/27/2010 | CLE | Ambriz | Demoted to reserve roster | | | Before |
| 9/27/2010 | CLE | Reynolds | Promoted to active roster | | | Before |
| 9/27/2010 | NYA | Carmona | Demoted to reserve roster | | | Before |
| 9/27/2010 | NYA | Dotel | Demoted to reserve roster | | | Before |
| 9/27/2010 | NYA | Guerra | Promoted to active roster | | | Before |
| 9/27/2010 | NYA | Halladay | Demoted to reserve roster | | | Before |
| 9/27/2010 | NYA | Hamilton | Demoted to reserve roster | | | Before |
| 9/27/2010 | NYA | Hernandez | Demoted to reserve roster | | | Before |
| 9/27/2010 | NYA | Holt | Promoted to active roster | | | Before |
| 9/27/2010 | NYA | Jeter | Demoted to reserve roster | | | Before |
| 9/27/2010 | NYA | Jones | Promoted to active roster | | | Before |
| 9/27/2010 | NYA | Mauer | Demoted to reserve roster | | | Before |
| 9/27/2010 | NYA | Miley | Promoted to active roster | | | Before |
| 9/27/2010 | NYA | Pujols | Demoted to reserve roster | | | Before |
| 9/27/2010 | NYA | Rivera | Demoted to reserve roster | | | Before |
| 9/27/2010 | NYA | Rodriguez | Demoted to reserve roster | | | Before |
| 9/27/2010 | NYA | Rodriguez | Promoted to active roster | | | Before |
| 9/27/2010 | NYA | Sabathia | Demoted to reserve roster | | | Before |
| 9/27/2010 | NYA | Stetter | Demoted to reserve roster | | | Before |
| 9/27/2010 | NYA | Triunfel | Promoted to active roster | | | Before |
| 9/27/2010 | NYA | Weathers | Promoted to active roster | | | Before |
| 9/29/2010 | KC | Lopez | Demoted to reserve roster | | | Before |
| 10/1/2010 | BOS | Cowgill | Released | | | Before |
| 10/1/2010 | BOS | Hill | Released | | | Before |
| 10/1/2010 | BOS | Hill | Demoted to reserve roster | | | Before |
| 10/1/2010 | DET | Beckett | Demoted to reserve roster | | | Before |
| 10/1/2010 | DET | Braden | Demoted to reserve roster | | | Before |
| 10/1/2010 | DET | Oswalt | Demoted to reserve roster | | | Before |
| 10/1/2010 | DET | Romo | Demoted to reserve roster | | | Before |
| 10/1/2010 | DET | Shields | Demoted to reserve roster | | | Before |
| 10/1/2010 | DET | Wieters | Demoted to reserve roster | | | Before |
| 10/1/2010 | SEA | Jackson | Demoted to reserve roster | | | Before |
| 10/1/2010 | SEA | Lowe | Demoted to reserve roster | | | Before |
| 10/1/2010 | SEA | Mickolio | Promoted to active roster | | | Before |
| 10/1/2010 | SEA | Rowland-Smith | Demoted to reserve roster | | | Before |
| 10/1/2010 | SEA | Sanchez | Demoted to reserve roster | | | Before |
| 10/1/2010 | TOR | Contreras | Demoted to reserve roster | | | Before |
| 10/1/2010 | TOR | Lee | Demoted to reserve roster | | | Before |
| 10/1/2010 | TOR | Meche | Demoted to reserve roster | | | Before |
| 10/1/2010 | TOR | Snell | Demoted to reserve roster | | | Before |
| 10/1/2010 | TOR | Uehara | Demoted to reserve roster | | | Before |
| 10/5/2010 | DET | Beckett | Promoted to active roster | | | Before |
| 10/5/2010 | DET | Braden | Promoted to active roster | | | Before |
| 10/5/2010 | DET | Cecil | Demoted to reserve roster | | | Before |
| 10/5/2010 | DET | Chirinos | Demoted to reserve roster | | | Before |
| 10/5/2010 | DET | Janish | Demoted to reserve roster | | | Before |
| 10/5/2010 | DET | Oswalt | Promoted to active roster | | | Before |
| 10/5/2010 | DET | Peralta | Demoted to reserve roster | | | Before |
| 10/5/2010 | DET | Rogers | Demoted to reserve roster | | | Before |
| 10/5/2010 | DET | Romo | Promoted to active roster | | | Before |
| 10/5/2010 | DET | Shields | Promoted to active roster | | | Before |
| 10/5/2010 | DET | Wieters | Promoted to active roster | | | Before |
| 10/5/2010 | DET | Wood | Demoted to reserve roster | | | Before |
| 10/5/2010 | NYA | Bruney | Demoted to reserve roster | | | Before |
| 10/5/2010 | NYA | Carmona | Promoted to active roster | | | Before |
| 10/5/2010 | NYA | Dotel | Promoted to active roster | | | Before |
| 10/5/2010 | NYA | Guerra | Demoted to reserve roster | | | Before |
| 10/5/2010 | NYA | Halladay | Promoted to active roster | | | Before |
| 10/5/2010 | NYA | Hamilton | Promoted to active roster | | | Before |
| 10/5/2010 | NYA | Hernandez | Promoted to active roster | | | Before |
| 10/5/2010 | NYA | Holt | Demoted to reserve roster | | | Before |
| 10/5/2010 | NYA | Igarashi | Demoted to reserve roster | | | Before |
| 10/5/2010 | NYA | Jeter | Promoted to active roster | | | Before |
| 10/5/2010 | NYA | Jones | Demoted to reserve roster | | | Before |
| 10/5/2010 | NYA | Mauer | Promoted to active roster | | | Before |
| 10/5/2010 | NYA | McAllister | Demoted to reserve roster | | | Before |
| 10/5/2010 | NYA | Miley | Demoted to reserve roster | | | Before |
| 10/5/2010 | NYA | Pujols | Promoted to active roster | | | Before |
| 10/5/2010 | NYA | Rivera | Promoted to active roster | | | Before |
| 10/5/2010 | NYA | Rodriguez | Promoted to active roster | | | Before |
| 10/5/2010 | NYA | Rodriguez | Demoted to reserve roster | | | Before |
| 10/5/2010 | NYA | Sabathia | Promoted to active roster | | | Before |
| 10/5/2010 | NYA | Stetter | Promoted to active roster | | | Before |
| 10/5/2010 | NYA | Triunfel | Demoted to reserve roster | | | Before |
| 10/5/2010 | NYA | Weathers | Demoted to reserve roster | | | Before |
| 10/5/2010 | SEA | Jackson | Promoted to active roster | | | Before |
| 10/5/2010 | SEA | Joyce | Demoted to reserve roster | | | Before |
| 10/5/2010 | SEA | Joyce | Promoted to active roster | | | Before |
| 10/5/2010 | SEA | Lowe | Promoted to active roster | | | Before |
| 10/5/2010 | SEA | Mickolio | Demoted to reserve roster | | | Before |
| 10/5/2010 | SEA | Rowland-Smith | Promoted to active roster | | | Before |
| 10/5/2010 | SEA | Sanchez | Promoted to active roster | | | Before |
| 10/5/2010 | TOR | Branyan | Demoted to reserve roster | | | Before |
| 10/5/2010 | TOR | Capuano | Demoted to reserve roster | | | Before |
| 10/5/2010 | TOR | Chen | Demoted to reserve roster | | | Before |
| 10/5/2010 | TOR | Choate | Demoted to reserve roster | | | Before |
| 10/5/2010 | TOR | Clemens | Demoted to reserve roster | | | Before |
| 10/5/2010 | TOR | Contreras | Promoted to active roster | | | Before |
| 10/5/2010 | TOR | Diamond | Demoted to reserve roster | | | Before |
| 10/5/2010 | TOR | Lee | Promoted to active roster | | | Before |
| 10/5/2010 | TOR | Meche | Promoted to active roster | | | Before |
| 10/5/2010 | TOR | Mulligan | Demoted to reserve roster | | | Before |
| 10/5/2010 | TOR | Snell | Promoted to active roster | | | Before |
| 10/5/2010 | TOR | Sonnanstine | Demoted to reserve roster | | | Before |
| 10/5/2010 | TOR | Uehara | Promoted to active roster | | | Before |
| 10/5/2010 | TOR | Wakefield | Demoted to reserve roster | | | Before |
